CLIA Reagents of DIABETES (CP)

【expected usage】

For in vitro quantitative detection of C-peptide (C-P) in human serum.

C-Peptide (C-P) is a connecting peptide connecting insulin A chain and B chain in the proinsulin molecule. It consists of 31 amino acid residues to form a connecting peptide with a molecular weight of 3021. Proinsulin is cleaved in the pancreas by proteases and carboxypeptidases to generate one molecule of insulin and one molecule of C-peptide.

C-peptide has no biological activity, is stored in secretory granules together with insulin, and is released from islet B cells to the blood in equimolar proportions together with insulin under glucose stimulation. Substances that stimulate or inhibit insulin secretion also stimulate C-peptide secretion. Under physiological conditions, C-peptide is mainly excreted by the kidneys.

Insulin in circulating blood is partially inactivated in the liver, so the measured plasma insulin cannot accurately reflect the secretion state of pancreatic islet B cells. Since C-peptide is produced in the process of insulin synthesis, its quantity has a parallel relationship with the secretion of insulin, and C-peptide antibodies do not cross-react with insulin, are not affected by exogenous insulin, and are hardly absorbed and absorbed by the liver. Metabolism, the half-life is more than 2 times longer than that of insulin, so the detection of serum C-peptide level can accurately reflect the secretory function of pancreatic islet B cells, and it is not affected by the production of insulin antibodies in the body after long-term insulin injection.

Since the 1970s, C-peptide has been used clinically to assess residual endogenous insulin secretion in diabetic patients. In addition, the determination of serum C-peptide content can also identify various causes of hypoglycemia and identify the type of diabetes.

At present, the commonly used clinical methods for C-peptide detection include enzyme-linked immunosorbent assay, fluorescence immunoassay, immunoturbidimetric assay, and chemiluminescence assay.

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