Chemiluminescence Reagent Kit Myoglobin MYO For Automatic Immunoassay Analyzer In Cardiac Marker

CLIA Reagents of Inflammation (MYO)

【Expected usage】
This product is used for the quantitative detection of myoglobin (MYO) in human serum in vitro.

Myoglobin (MYO), a protein that carries oxygen in muscles, consists of 153 amino acid residues, contains heme, is homologous to hemoglobin, and has a binding capacity between hemoglobin and cytochrome enzymes. Helps muscle cells transport oxygen to mitochondria. Its essence is a binding protein composed of a peptide chain and a heme prosthetic group.

Detection of serum myoglobin can be used as the most sensitive indicator for the diagnosis of acute myocardial infarction (AMI). However, the specificity is poor, and diseases such as skeletal muscle injury, trauma, and renal failure can lead to its increase. Although Myo positive cannot diagnose AMI, it can be used as an important indicator for early diagnosis of AMI. For example, if Myo is negative, myocardial infarction is basically excluded, and it can also be used for the diagnosis of reinfarction. Combined with clinical, if Myo is re-increased, it should be considered as reinfarction. or infarct extension. It can be used for early diagnosis of acute myocardial infarction (AMI).

The current clinical methods for the detection of myoglobin (MYO) include radioimmunoassay, enzyme-linked immunosorbent assay, colloidal gold method, immunoturbidimetry and chemiluminescence immunodiagnosis.

【Inspection Principle】
This kit adopts the principle of direct sandwich method, uses magnetic microparticles as the solid phase of immune reaction, and uses chemiluminescence enzyme-linked immunosorbent assay method to cooperate with chemiluminescence measuring instruments to detect the content of MYO in human serum.

The technical principle is: Fluorescein isothiocyanate (FITC)-labeled mouse monoclonal anti-MYO antibody and alkaline phosphatase (AP)-labeled mouse monoclonal anti-MYO paired antibody and samples, calibrators or quality controls. The MYOs combine to form a "sandwich" complex. Then, the magnetic particles linked with goat anti-FITC antibody are added, and the antigen-antibody immune complex is bound to the magnetic particles through the specific binding of the anti-FITC antibody and FITC.

Under the action of an external magnetic field, the complex formed by the immune reaction is separated from other unbound substances, and after washing the complex, an enzymatic chemiluminescence substrate is added. The substrate is catalytically cleaved under the action of the enzyme to form an unstable excited state intermediate.

When the excited state intermediate returns to the ground state, photons are emitted to form a luminescence reaction, and the luminescence intensity of the reaction can be detected by a chemiluminescence instrument. In the detection wavelength range (230-700nm), the luminescence intensity is proportional to the content of MYO in the sample, and the MYO concentration in the sample can be calculated by fitting the modified four-parameter Logistic equation.