Chemiluminescencross In Vitro Diagnostic Reagents HFABP Heart Fatty Acid Binding Protein

CLIA Reagents of Inflammation (HFABP)

【Expected usage】

This product is used to quantitatively detect the content of human serum central fatty acid binding protein (H-FABP) in vitro. Heart-type fatty acid-binding protein (H-FABP) is a novel small cytoplasmic protein abundant in the heart. It is highly cardiac-specific (ie, expressed primarily in cardiac tissue), but is also expressed at low concentrations in tissues other than the heart. Following myocardial ischemic injury, H-FABP can be detected in the blood as early as 1-3 hours after the onset of chest pain, peaking at 6-8 hours and plasma levels returning to normal within 24-30 hours.

The cardiac fatty acid-binding cytoplasmic protein consists of 132 amino acids with a molecular weight of 15 kDa. The heart-shaped fatty acid binding protein (H-FABP) gene is located on chromosome I. It is one of the most abundant proteins in the heart. H-FABP binds two fatty acid molecules and participates in the transport of fatty acyl-CoA, is active in the oxidation process, and thus in Energy is produced in mitochondria.
Heart-type fatty acid-binding protein is currently measured in clinical and laboratory methods including enzyme-linked immunosorbent assay, colloid Gold method, fluorescence immunoassay, chemiluminescence method, etc.

【Inspection Principle】

This kit adopts the principle of direct sandwich method, uses magnetic microparticles as the solid phase of immunoreaction, and uses chemiluminescence enzyme-linked immunoassay method and chemiluminescence measuring instrument to detect the content of H-FABP in human serum.

The technical principle is: Fluorescein isothiocyanate (FITC)-labeled mouse monoclonal anti-H-FABP antibody paired with alkaline phosphatase (AP)-labeled mouse monoclonal anti-H-FABP antibody and samples, calibrators or The H-FABP in the control binds to form a "sandwich" complex. Subsequently, the magnetic particles linked with goat anti-fluorescein isothiocyanate (FITC) antibody were added, and the antigen-antibody immune complexes were bound to the magnetic particles through the specific binding of the anti-FITC antibody to FITC.

Under the action of an external magnetic field, the complex formed by the immune reaction is separated from other unbound substances, and after washing the complex, an enzymatic chemiluminescent substrate (adamantane derivative) is added. The substrate is catalytically cleaved under the action of the enzyme to form an unstable excited state intermediate.

When the excited state intermediate returns to the ground state, photons are emitted to form a luminescence reaction, and the luminescence intensity of the reaction can be detected by a chemiluminescence instrument. In the range of measurement wavelength (230-700nm), the luminescence intensity is proportional to the content of H-FABP in the sample, and the H-FABP concentration in the sample can be calculated by fitting the modified four-parameter Logistic equation.