Cholyglycine CG In Vitro Diagnostic Reagents For Automatic Immunoassay Analyzer In Hepatic Fibrosis

CLIA Reagents of Inflammation (CG)

【Expected usage】
This product is used to quantitatively detect the content of glycocholic acid (CG) in human serum in vitro.

Glycocholic acid is a conjugated cholic acid composed of cholic acid and glycine, and is one of the main components of bile acid. Cholesterol undergoes an extremely complex enzymatic reaction in hepatocytes and is converted into primary bile acids, including cholic acid (CA) and chenodeoxycholic acid (CDCA). When the liver cells are damaged and the liver is diseased, the metabolism and circulation of glycocholic acid will be disturbed, and the amount of hepatic bile acid in serum will increase, which is higher than that of ALT, AST, total bilirubin (TBIL), alkaline phosphatase (ALP). ), glutyl transpeptidase (GGT) and serum albumin (ALB) and other conventional liver function indicators are more sensitive. Patients with various liver diseases, such as cirrhosis, acute hepatitis, chronic hepatitis, etc., have significantly elevated glycocholic acid levels, so glycocholic acid can be used as a good indicator for the diagnosis of these diseases.

At present, the clinical detection of glycocholic acid (CG) content mainly adopts radioimmunoassay, enzyme immunoassay, chemiluminescence method, colloidal gold method and immunoturbidimetric method.

【Inspection Principle】
This kit uses magnetic microparticles as the solid phase of the immune reaction, and uses the chemiluminescence enzyme-linked immunosorbent assay method to cooperate with the chemiluminescence measuring instrument to detect the CG content in human serum.

The technical principle is: glycocholic acid in the test sample, calibrator or quality control product competes with the alkaline phosphatase (AP)-labeled CG derivative in anti-reagent A to bind to fluorescein isothiocyanate in anti-reagent B. (FITC)-labeled mouse monoclonal anti-CG antibody, followed by the addition of a magnetic particle reagent linked with goat anti-FITC antibody, the antigen-antibody immunocomplexed by the specific binding of goat anti-FITC antibody in the magnetic particle reagent to FITC in anti-reagent B bound to the magnetic particles.

Under the action of an external magnetic field, the complex formed by the immune reaction is separated from other unbound substances, and after washing the complex, an enzymatic chemiluminescence substrate is added. The substrate is catalytically cleaved under the action of the enzyme to form an unstable excited state intermediate.

When the excited state intermediate returns to the ground state, photons are emitted to form a luminescence reaction, and the luminescence intensity of the reaction can be detected by a chemiluminescence instrument. Within the detection range, the luminescence intensity is inversely proportional to the content of CG in the sample, and the CG concentration in the sample can be calculated by fitting the modified four-parameter Logistic equation.