Stainless Steel Biosafe Laminar Flow Cabinet 0.45m/s Air Velocity

Biological Safety Cabinet (BSC) Core Functions & Classification

Biological Safety Cabinets provide ‌triple containment‌ (operator/sample/environment) via ‌HEPA filtration systems‌ and ‌negative-pressure engineering‌‌. Primary classifications per international standards:

1. ‌Class I‌: Basic operator & environmental protection; suitable for low-risk agents (e.g., bacterial cultures)‌
2. ‌Class II (Most Common)‌:
   • ‌Type A2‌: 70% air recirculation + 30% exhaust; for microbiological work/pathogenic agents‌
   • ‌Type B2‌: 100% hard-ducted exhaust; for bioassays with volatile chemicals/radionuclides‌
3. ‌Class III‌: Totally enclosed; for high-risk pathogens (e.g., Ebola virus)‌

Standard Operating Protocols

1. ‌Start-up‌:
   • Raise sash to marked height; pre-run ≥10 mins for airflow stabilization‌
   • Arrange equipment sequentially: clean zone → work zone → contaminated zone‌
2. ‌Operations‌:
   • Insert arms slowly; pause 1 min for airflow recovery‌
   • Avoid obstructing grilles; segregate items by "dry→wet" and "clean→contaminated"‌
3. ‌Shutdown‌:
   • Remove materials → disinfect interior surfaces → run 3 mins to purge particulates‌
   • Close sash → deactivate blower/lights → initiate UV sterilization ≥30 mins‌

️ Critical Compliance Notes

• ‌Prohibited Uses‌:
  • Never handle explosives or strong volatile acids (exceeds HEPA capacity)‌
  • BSCs ≠ Laminar Flow Hoods (latter lack operator protection)‌
• ‌Maintenance Requirements‌:
  • Monthly airflow verification (Type A2 inflow velocity ≥0.50 m/s)‌
  • HEPA filter replacement every 1–2 years + mandatory ‌KI-Discus leak testing‌ (sodium iodide aerosol challenge)‌