EDTA Blood Collection Tube for Liquid Biopsy with Cell Protection
I. Product Features
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Precision Anticoagulation Mechanism
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Utilizes potassium ethylenediaminetetraacetate (EDTA-K₂ or EDTA-K₃) to inhibit coagulation by chelating calcium ions, preserving blood cell morphology and quantitative integrity.
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Strict anticoagulant dosage of 1.5–2.2 mg/mL blood, ensuring optimal anticoagulation without under- or over-treatment.
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Premium Materials & Craftsmanship
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Tube Material: High-clarity medical-grade PET plastic (lightweight, shatter-resistant) or glass (for large volumes), with silicone-coated interior to prevent cell adhesion and hemolysis.
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Sealing System: Butyl rubber stopper offers superior airtightness (>40,000 puncture tolerance), minimizing needle wear.
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Vacuum Accuracy: Imported equipment guarantees blood draw volume error ≤±5% (e.g., 1.9–2.1 mL for 2 mL tubes).
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Standardization & Safety
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Purple cap (universal color code) for rapid identification.
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Gamma-irradiated sterilization, endotoxin-compliant, certified to ISO 13485 and CE standards.
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II. Application Scenarios
| Test Type | Specific Applications | Mechanism |
|---|---|---|
| Basic Hematology | Complete Blood Count (CBC), blood typing, erythrocyte sedimentation rate (requires dedicated tube) | Preserves cell morphology, prevents clotting interference |
| Specialized Tests | HbA1c, flow cytometry, pathogen nucleic acid testing (PCR) | Stabilizes RBC membranes, protects DNA/RNA integrity |
| Contraindications | Coagulation tests (PT/APTT), calcium/potassium ion assays, trace element analysis | EDTA chelation disrupts ion balance and clotting factor activity |
III. Key Specifications (Table)
| Parameter | K2/K3 EDTA Tube Specifications | Remarks |
|---|---|---|
| Volume Options | 1-10ml (PET/GLASS) | Accommodates diverse sample volume needs |
| Anticoagulant Conc. | 1.5–2.2 mg/mL blood | <1.5 mg/mL risks clotting; >2.2 mg/mL shrinks cells |
| Centrifugation | 3500–4000 rpm × 5 min | Separates plasma/cellular layers |
IV. Precautions
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Collection Protocol
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Gently invert tubes 5–10 times immediately after draw—do not shake—to prevent microclots.
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Fill to marked volume: underfilling causes partial clotting; overfilling dilutes anticoagulant (causing pseudothrombocytopenia).
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Sample Handling & Storage
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Time Sensitivity: Prepare blood smears within 2 hours; whole blood stored ≤6 hrs (RT) or ≤4 hrs (4°C)—prolonged storage alters cell morphology.
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Temperature: Ideal testing range: 18–22°C; extremes (>30°C or <15°C) affect cell volume.
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Order of Draw
Follow sequence: Blood culture → Plain tubes → Citrate coagulation tubes → EDTA tubes to avoid cross-contamination. -
V. Frequently Asked Questions (FAQs)
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Microclots observed post-collection. Solution?
Cause: Inadequate/untimely mixing (especially at >25°C) or underfilled tube.
Action: Invert ≥8 times; ensure precise fill volume. -
EDTA-K₂ vs. EDTA-K₃: Key differences?
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Form: K₂ = crystals; K₃ = liquid spray (faster dissolution).
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Impact: K₃ overuse dilutes samples (falsely lowers Hb); K₂ offers better batch consistency.
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Suitable for coagulation tests?
No! EDTA chelates calcium, disrupting coagulation cascades. Use dedicated citrate tubes (blue cap) instead. -
Causes/prevention of hemolysis?
Causes: Blood impacting tube bottom, violent mixing, premature refrigeration (<30 min post-draw).
Prevention: Angle tubes to let blood flow along walls; use large-bore needles; invert gently. -
Preventing blood reflux infection?
Procedure: After draw → Remove tube first → Release tourniquet → Withdraw needle. Prevents negative-pressure backflow.
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