China EDTA Blood Collection Tube with Precise Anticoagulation for sale

EDTA Blood Collection Tube with Precise Anticoagulation

Price Negotiable

Price: Negotiation

MOQ: 100/pcs

Delivery Time: 1-4 weeks

Brand: Orsin

Product Description

I. Product Characteristics

EDTA blood collection tubes (purple cap) utilize dipotassium or tripotassium ethylenediaminetetraacetate (EDTA-K₂ or EDTA-K₃) as the core anticoagulant, featuring:

Precise Anticoagulation Mechanism: Chelates calcium ions (Ca²⁺) in blood to block thrombin activation, achieving complete anticoagulation while maximally preserving blood cell morphology.
Optimized Physical Design:
- High-transparency PET plastic or glass tube with mechanical strength and light resistance;
- Pre-filled precise anticoagulant dosage (1.5–2.2 mg EDTA/mL blood), vacuum accuracy ≤±5%.
Safety & Quality Control:
- Gamma-irradiated sterilization (8–25 kGy), controlled endotoxin levels;
- ISO13485/CE compliant; standardized purple cap for rapid identification.

II. Application Scenarios

Primarily for hematological testing, with specific applications:

Test Category	Suitable Applications	Restrictions
Basic Hematology	Complete Blood Count (CBC), Hemoglobin (Hb), Blood Typing, Reticulocyte Count	Coagulation Tests (e.g., PT/APTT), Platelet Function Testing
Specialized Tests	Glycated Hemoglobin (HbA1c), Hemoglobin Electrophoresis, Flow Cytometry	Electrolytes (Ca²⁺/K⁺/Na⁺)/Iron Assays, Alkaline Phosphatase, Creatine Kinase
Molecular Biology	DNA/RNA Extraction, Pathogen Nucleic Acid Testing (with stabilizers)	PCR (EDTA may inhibit enzyme activity)

Note: EDTA tubes cannot be used for electrolyte, trace element, or coagulation testing due to chelation interference.

III. Usage Protocol & Specifications

Procedure	Requirements	Key Operational Notes
Pre-collection	Check expiration date/vacuum integrity	Storage: 2–25°C, dark, dry conditions
Collection & Mixing	Gently invert 5–8 times immediately post-draw	Excessive force → hemolysis; insufficient mixing → microclots; accelerate if >25°C
Testing Window	Routine tests: complete within 24h	WBC count: 48h at 4°C; Platelet count: ≤24h at 4°C
Centrifugation	Not for direct centrifugation	Plasma separation requires gel separator (0.8–1.2 g/tube)

IV. Precautions

Anticoagulant Risks:
- Overfill (>2.2 mg/mL): RBC shrinkage/platelet swelling → morphology distortion;
- Underfill: Inadequate anticoagulation → microclots → instrument obstruction.
Sample Handling:
- Do not reuse tubes or mix additives;
- Blood volume ≤ labeled capacity (e.g., ≤5.5 mL for 5 mL tube).
Safety:
- EDTA is corrosive; rinse skin/eyes immediately with water if contacted.

V. FAQs

EDTA-K₂ vs. EDTA-K₃?
- Solubility: K₃ dissolves easier → liquid pre-filled tubes; K₂ → spray-dried plastic tubes;
- Performance: Comparable anticoagulation, but K₃ excess may cause platelet satellitism (pseudothrombocytopenia).
Causes of Pseudothrombocytopenia? Solutions?
- Causes: EDTA-induced platelet aggregation (~0.1% incidence) or neutrophil adhesion (satellitism);
- Resolution: Recollect using sodium citrate tube.
Can tubes with microclots be used?
→ No. Microclots indicate anticoagulation failure → recollect with thorough mixing.
Suitable for glucose testing?
→ Compatible with sodium fluoride (2–4 mg/mL blood) to inhibit glycolysis; validate system compatibility.

Clinical Note: EDTA tubes remain the gold standard for CBC. For abnormal cases (e.g., suspected platelet disorders), parallel testing with alternative anticoagulants is recommended.