Liquid Biopsy-Specific EDTA Blood Tube with Enhanced Cell Protection and Plastic Cap
I. Product Core Feature
II. Application Scenarios
| Test Type | Specific Applications | Mechanism |
|---|---|---|
| Basic Hematology | Complete Blood Count (CBC), blood typing, erythrocyte sedimentation rate (requires dedicated tube) | Preserves cell morphology, prevents clotting interference |
| Specialized Tests | HbA1c, flow cytometry, pathogen nucleic acid testing (PCR) | Stabilizes RBC membranes, protects DNA/RNA integrity |
| Contraindications | Coagulation tests (PT/APTT), calcium/potassium ion assays, trace element analysis | EDTA chelation disrupts ion balance and clotting factor activity |
III. Key Specifications (Table)
| Parameter | K2/K3 EDTA Tube Specifications | Remarks |
|---|---|---|
| Volume Options | 1-10ml (PET/GLASS) | Accommodates diverse sample volume needs |
| Anticoagulant Conc. | 1.5–2.2 mg/mL blood | <1.5 mg/mL risks clotting; >2.2 mg/mL shrinks cells |
| Centrifugation | 3500–4000 rpm × 5 min | Separates plasma/cellular layers |
IV. Precautions
- Storage Requirements
- Store in a dry, cool environment at 2–25℃; avoid direct sunlight, high temperature (above 30℃) and humidity (relative humidity >80%) to prevent degradation of the cell protection formula and anticoagulant.
- Do not freeze the product. Freezing may cause tube breakage or damage to the inner coating, affecting sample stability.
- Keep the tubes in their original packaging until use to avoid contamination and light exposure.
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V. Frequently Asked Questions (FAQs)
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Microclots observed post-collection. Solution?
Cause: Inadequate/untimely mixing (especially at >25°C) or underfilled tube.
Action: Invert ≥8 times; ensure precise fill volume. -
EDTA-K₂ vs. EDTA-K₃: Key differences?
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Form: K₂ = crystals; K₃ = liquid spray (faster dissolution).
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Impact: K₃ overuse dilutes samples (falsely lowers Hb); K₂ offers better batch consistency.
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Suitable for coagulation tests?
No! EDTA chelates calcium, disrupting coagulation cascades. Use dedicated citrate tubes (blue cap) instead. -
Causes/prevention of hemolysis?
Causes: Blood impacting tube bottom, violent mixing, premature refrigeration (<30 min post-draw).
Prevention: Angle tubes to let blood flow along walls; use large-bore needles; invert gently. -
Preventing blood reflux infection?
Procedure: After draw → Remove tube first → Release tourniquet → Withdraw needle. Prevents negative-pressure backflow.
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