Plasma Recombinant Holo Transferrin TF Iron Binding Protein Pichia Pastoris Expressed
Recombinant Holo Transferrin Animal-Free Pichia pastoris Expressed
Transferrin (TF) is the major iron-binding protein in plasma. It is not only involved in iron transport and metabolism, as well as the regulation of respiration, cell proliferation and the immune system, but also exerts antioxidant and bactericidal functions, with a wide range of applications in cell culture.
Transferrin exists in three forms: apo-transferrin (iron-free, not bound to iron ions), holo-transferrin (iron-saturated, bound to two iron ions) and partially saturated transferrin.
Anrate Recombinant Holo-Transferrin is a recombinant transferrin expressed and purified from Pichia pastoris via genetic recombination technology. It contains no animal-derived components, carries no risk of blood-borne virus infection, and features high batch-to-batch consistency.
As an iron-saturated holo-transferrin product, it not only performs the function of iron transport, but also serves as an iron source for cells by itself, and is suitable for animal cell culture.
Product Source
Expressed in recombinant Pichia pastoris
Specifications parameters
| CAS | 11096-37-0 |
| MDL | MFCD00131337 |
| Source | Pichia pastoris |
| Purity | ≥98.0% (HPLC-BR) |
| Appearance | Salmon pink to red loose body or powder |
| Identification | Positive(Western blotting) |
| Protein content | 95% - 105.0% |
| Molecular weight | 75.2 kD ± 7.5 kD (SDS-PAGE) |
| Loss on Drying | ≤8.0% |
| pH | 7.0 - 9.0 |
| Iron content | 0.10% - 0.35%(AAS) |
| Endotoxin | ≤ 0.5 EU/mg (The bacterial endotoxin limit should be less than 0.5 EU/mg) |
Reconstitution Method
It is recommended that the final concentration does not exceed 50 mg/ml. For reconstitution, dissolve the lyophilized powder in sterile PBS or other suitable solvent, then filter through a sterile filter membrane. The solution can be further diluted and aliquoted with other solvents per customer requirements. The sterile solution is stable for 30 days at 2–8 °C. Once opened, use promptly to avoid contamination.
Storage Conditions
Store this product at 2–8 °C, protected from light and moisture, in a tightly sealed and dry condition. For unopened products, the validity period complies with the stated shelf life. After opening, use promptly and ensure the container is resealed after each use. Avoid freeze-thaw cycles, which may impair the performance of the culture medium.
Shelf Life
The tentative shelf life of this product is 5 years.
Disclaimer
This product is intended exclusively for scientific research experiments, laboratory investigation and industrial production purposes. It is strictly prohibited for use in clinical therapeutic applications, and must not be administered to animals or human beings.
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