Human Recombinant Holo Transferrin Cell Culture Animal Free Mammalian Cell Culture
ART202S Recombinant Holo Transferrin Animal Free Mammalian Cell Culture
Animal-Free, Pichia pastoris Expressed For Mammalian Cell Culture
Iron delivery is a foundational requirement for mammalian cell growth and recombinant protein expression. Traditional plasma-derived transferrin carries inherent risks of blood-borne contaminants, batch-to-batch variability and regulatory hurdles, making it a bottleneck for serum-free media development and biopharmaceutical scale-up.
Anrate ART202S recombinant holo-transferrin is a high-purity, animal-free iron transport protein produced in a Pichia pastoris expression system. As a fully iron-saturated holo form, it delivers bioavailable iron directly to cells via transferrin receptors, serving as a safe, consistent and regulatory-friendly replacement for plasma-derived transferrin in serum-free and chemically defined cell culture workflows.
Key Advantages
I.100% Animal-Free, Zero Blood-Borne Risk
Expressed in recombinant Pichia pastoris with no animal-derived raw materials throughout the manufacturing process. Eliminates the risk of viral, mycoplasma and prion contamination, and supports compliance with FDA, EMA and ICH guidelines for biopharmaceutical development.
II.Pre-Saturated Holo Form, Ready to Use
Each transferrin molecule binds two ferric ions at full saturation. No additional iron chelation is required, simplifying media formulation and reducing process variables in mammalian cell culture.
III.High Purity & Ultra-Low Endotoxin
Purified via multi-step chromatography with ≥98% homogeneity. Endotoxin level is controlled below 0.5 EU/mg, minimizing cytotoxicity and inflammatory response, and ensuring compatibility with sensitive cell lines, stem cells and immune cell culture.
IV.Superior Batch-to-Batch Consistency
Standardized fermentation and purification processes guarantee stable protein concentration, iron saturation and bioactivity across production lots. Reliable batch consistency reduces experimental variation and supports reproducible biomanufacturing processes.
V.Broad Mammalian Cell Compatibility
Validated for use with CHO, HEK293, Vero, BHK, hybridoma, mesenchymal stem cells and iPSCs. Suitable for both basic research and industrial-scale bioproduction of antibodies, recombinant proteins and vaccines.
VI.Scalable Packaging, Global Supply
Available in 1 g, 10 g and 100 g pack sizes, with custom specifications on request. Stable production capacity and worldwide logistics support customers across North America, Europe and Asia-Pacific.
Technical Specifications
| Parameter | Specification |
| Catalog No. | ART202S |
| Product Name | Recombinant Holo-Transferrin |
| Expression System | Pichia pastoris (recombinant) |
| Purity | ≥ 98.0% (HPLC) |
| Endotoxin | ≤ 0.5 EU/mg (LAL assay) |
| Iron Content | 0.10% – 0.35% |
| Form | Lyophilized powder, pink appearance |
| Recommended Reconstitution | ≤ 50 mg/ml in sterile PBS |
| Storage | 2–8 °C, protected from light, sealed |
| Shelf Life | 5 years under unopened conditions |
Typical Applications
- Serum-Free & Chemically Defined Media Formulation: Replace plasma-derived transferrin to build fully animal-free culture systems for antibody, vaccine and cell therapy development.
- Biopharmaceutical Manufacturing: Support high-density suspension culture of CHO and HEK293 cells, enhancing cell proliferation and target protein expression with consistent batch quality.
- Stem Cell & Immune Cell Culture: Low-endotoxin, xeno-free formulation meets the high safety and consistency requirements of MSC, CAR-T and iPSC cultivation.
- In Vitro Diagnostics & Basic Research: Used as a raw material in cell assay kits and for fundamental cell biology research.
Why Choose Anrate?
Anrate specializes in recombinant protein R&D and manufacturing with a proprietary Pichia pastoris expression platform. We control the entire production chain from gene construction to final fill-finish, ensuring stable supply, competitive pricing and full traceability. Every batch is released with a comprehensive Certificate of Analysis (CoA), and our technical team provides application support for media optimization and process scale-up.
Disclaimer
This product is intended exclusively for scientific research experiments, laboratory investigation and industrial production purposes. It is strictly prohibited for use in clinical therapeutic applications, and must not be administered to animals or human beings.
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