100ml HSA Cell Culture Grade Lyophilized Recombinant Albumin Powder CAS 70024-90-7
HSA Cell Culture Grade Lyophilized Recombinant Albumin Powder
CAS Number: 70024-90-7 | Brand: Anrate | Catalog No.: ART103S
Product Introduction
Anrate cell culture grade recombinant human albumin (rHSA) is a high-performance cell culture medium supplement engineered specifically for serum-free and chemically defined culture systems. Expressed in Pichia pastoris via recombinant DNA technology and purified through multi-step chromatography, this lyophilized powder delivers a consistent, animal-origin-free alternative to plasma-derived HSA and bovine serum albumin (BSA).
With precisely controlled quality specifications and low endotoxin levels, it supports robust cell growth, maintains cell viability and morphological uniformity, and minimizes experimental variation caused by batch-to-batch differences in serum-derived products. It is widely adopted in academic research, cell therapy development and biopharmaceutical production workflows.
Key Benefits for Cell Culture
Optimized for Serum-Free Systems
Formulated to support adhesion, proliferation and expansion of mammalian cells. Serves as a core nutrient carrier and osmotic stabilizer in chemically defined media, enabling reliable serum-free culture without performance compromise.
100% Animal-Origin-Free Process
Entire manufacturing chain uses no animal-derived raw materials. Eliminates risks of blood-borne pathogens, mycoplasma and xenogeneic protein interference, simplifying regulatory documentation for cell therapy and biologic development projects.
Ultra-Low Impurity Profile
≥99% purity verified by SDS-PAGE and HPLC, with endotoxin ≤0.5 EU/mg and minimal host cell residual. The clean formulation reduces cytotoxic interference and background noise in downstream assays.
Superior Batch-to-Batch Consistency
Industrial-scale fermentation and standardized purification ensure highly consistent product quality across production lots. This eliminates experimental variability caused by serum albumin batch differences and improves data reproducibility.
Easy-Use Lyophilized Format
Porous lyophilized cake dissolves rapidly at room temperature into a clear, particle-free solution. Ready for direct addition to culture media, suitable for both bench-scale research and large-volume bioproduction preparation.
Product Specifications
| Parameter | Specification |
| CAS Number | 70024-90-7 |
| Expression Host | Recombinant Pichia pastoris |
| Appearance | Off-white to white lyophilized powder or cake |
| Purity | ≥99.0% (SDS-PAGE & HPLC) |
| Molecular Weight | ~66.5 kDa |
| Endotoxin | ≤0.5 EU/mg |
| Host Cell Protein | ≤10 ng/g |
| pH | 6.4 – 7.4 |
| Protein Content | 95.0% – 110.0% |
Application Areas
Serum-Free Cell Culture Media
Core additive for chemically defined and xeno-free media formulations, supporting routine culture of CHO, HEK293, VERO and other production cell lines.
Stem Cell & Immune Cell Research
Suitable for expansion of mesenchymal stem cells (MSCs), T cells, NK cells and iPSCs, helping maintain cell phenotype and differentiation potential during long-term culture.
Cell Cryopreservation Formulations
Key protective component in serum-free freezing media, reducing ice crystal damage and improving post-thaw cell recovery rate and viability.
Biologic R&D Excipient
Used as a stabilizer and protectant in protein reagent, antibody and vaccine formulation development, preventing protein aggregation and denaturation during storage.
In Vitro Diagnostic Assays
Blocking reagent and protein stabilizer for ELISA, lateral flow assays and Western blotting, reducing non-specific binding and improving assay precision.
Protein Storage & Handling
Protects recombinant proteins, enzymes and antibodies from surface adsorption and degradation during dilution, storage and transportation.
Reconstitution Procedure
Quality Commitment
Frequently Asked Questions
Q1: What makes this "cell culture grade" recombinant HSA different from general-grade rHSA?
A1: Cell culture grade rHSA undergoes additional cell compatibility validation and tighter impurity controls, including lower endotoxin and residual host protein specifications. It is specifically qualified to support cell growth and viability without cytotoxic effects, making it suitable for sensitive cell culture applications.
Q2: Can this product replace BSA or plasma-derived HSA in my existing culture protocol?
A2: Yes. Anrate recombinant HSA matches native human albumin in structure and biological function. It can typically replace BSA or plasma-derived HSA at an equivalent ratio in most culture protocols, while eliminating animal-derived contaminants and batch variation.
Q3: What is the recommended working concentration in cell culture media?
A3: A working concentration of 0.5–2 g/L is typical for most mammalian cell culture systems. Optimal dosage varies by cell type and culture objective; we recommend titration within your specific system.
Q4: Do you offer bulk packaging for bioproduction scale use?
A4: Yes. We provide kilogram-scale and custom bulk packaging to support pilot-scale and industrial bioproduction. Contact our sales team for bulk pricing and lead time information.
Q5: Does this product contain any animal-derived components?
A5: No. The entire manufacturing process, from raw materials to final purification, uses no animal-origin inputs. The product is fully xeno-free and suitable for projects requiring animal-component-free documentation.
Important Notice
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