Male Infertiloty Test Sperm Function Analysis Sperm Reactive Oxygen Species (ROS) Assay Kit (NBT Method)

The kit is used for quantitative detection of sperm reactive oxygen

species levels in adult males.

Clinical Significance

• In normal physiological condition, a balance exists between ROS generation and scavenging. Limited amounts of ROS is needed to regulate normal sperm function. ROStakes an important role in sperm capacitation, sperm-oocyte fusion and etc.
• If ROS production exceeds the body's purification ability, the ROS accumulation can lead to oxidative stress injury (such as peroxidation of lipids, sperm DNA and mitochondria damage, cellapoptosis), causing sperm function and vitality damage, thus negatively affect fertility.

Technical Advantages

• The detection substance of this kit is the activity of superoxide anion radical of sperm, rather than the product of oxidative stress injury(such as MDA or LPO)or the indirect substances involved in ROS metabolizing enzymes(such as SOD and GSH-Px,)so it can reflect the true level of ROS more intuitively.
• In good relevance with the results of chemiluminescence method
• No special instruments needed. Easy to operate. Batch automatic tests could be available with the helpof Chemwell BRED automatic chemical-Immune analyzer. It could be also manually operated by microplate reader (with 630-nm wavelength).

Applicable Conditions

• Increased sperm DNA fragmentation
• Asthenospermia
• Sperm dysmaturity
• Abnormal sperm morphology
• Sperm fertilization dysfunction
• Evaluation of the sperm preparation effect

Specimen:

• sperm

Specification:

• 20 tests/kit

Applicable Instrument

• Chemwell BRED automatic chemical-Immune analyzer,
• Automatic chemical-Immune analyzer,
• Microplate reader