DHE Staining for ROS Flow Cytometry

Sperm superoxide anion (O2-.) detection

Sperm Reactive Oxygen Species DHE Staining kit

Reactive Oxygen Species can be induced by some stress conditions like exposure to oxidant or drugs. This fact leads to oxidative

stress. ROS induce damage in DNA, protein and lipids with important consequences in cells. Sperm Reactive oxygen species DHE

staining kit is useful for the detection of reactive oxygen species (ROS) in sperm.

Aplications: 

• Analyze the etiology of infertility.

• Analyze the reasons of ART adverse outcomes (low fertilization and blastocyst formation rate, miscarriage).

• Analyze the reasons of low quality semen parameters.

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Features:

• Specific targeting probe for superoxide anion, with an accurate result.

• Exclude the interference of dead sperm, and report the mitochrondrial ROS level in live sperm, which is more clinically meaningful.

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  A large number of sperm can be easily analyzed, leading to good repeatable results.

• Required device: flow cytometer (blue excitation).

   

 Test results:

                   Identify and pick live sperm                                       Label O₂^-.