Rapid ROS test , Seminal reactive oxygen species staining kit (NBT method)

The experimental operation is simple and fast. It is equipped with a special reaction tube. Just add an appropriate amount

of sperm into the reaction tube, add 100ul NBT solution, and then add NBT buffer to the red scale line. Mix it well. After

incubation for 30 minutes, observe the results with naked eyes.

Clinical significance:

This test is mainly used for the etiological analysis and prognosis monitoring of male infertility. it is also used to evaluate the

level of sperm reactive oxygen species in male infertile patients, and the effect of antioxidant treatment.

Test principle:

The superoxide ions of sperm ROS have high reactivity, which can be reduced by NBT to blue formazan. The color of formazan

is positively correlated with the content of O2- in semen.

Features:

• It does not need other special equipment. It completes the test in 30 minutes (the results are easy to interpret, and the results are observed by the naked eye through the reaction color).
• The operation is simple, and it could be used as semen routine assay in all andrology labs.
• Only a small amount of semen is needed to detect whether the patient has oxidative damage.
• The test results are consistent with clinical symptoms.