Bone Peptone (Biological Grade) — High Nitrogen ≥ 14.5%, Low Ash ≤ 5.0%, Excellent Clarity for Microbial Culture
Bone Peptone (Biological Grade) — High Nitrogen, Low Ash, Preferred Raw Material for Microbial Culture
Product Introduction
Bone Peptone is a high‑nitrogen, low‑ash, high‑clarity peptone product made from fresh bovine bone through pancreatic enzymolysis, low‑temperature extraction, refining, and spray drying.
It is rich in peptides, amino acids, and trace elements, and is especially suitable for high‑end biological applications such as biopharmaceutical fermentation, microbiological media production, vaccine manufacturing, and diagnostic reagents.
Raw Material & Process
| Item | Description |
|---|---|
| Raw Material Source | Fresh bovine bone (pollution‑free, no spoilage) |
| Production Process | Washing & sterilization → Enzymolysis → Separation → Decolorization & deodorization → Refined filtration → Ultrafiltration → Concentration → Spray drying |
| Process Features | Low‑temperature enzymolysis preserves active components; ultrafiltration ensures clarity; spray drying guarantees stability |
| Applicable Standards | Complies with Chinese Biological Products Main Raw and Auxiliary Materials Quality Control Standard (2000 edition) and enterprise standard Q/SCSH 001‑2025 |
Product Features
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High Total Nitrogen ≥ 14.5% – Provides sufficient nitrogen source to promote rapid microbial growth
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Amino Nitrogen ≥ 2.5% – Rich in small peptides and free amino acids, easily utilized by microorganisms
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Low Ash ≤ 5.0% – Reduces impurity interference, suitable for precision fermentation
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Excellent Clarity – 2% aqueous solution remains clear and precipitate‑free after heating and cooling, ideal for high‑purity media preparation
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Stable Biological Performance – Verified with multiple standard strains, supporting tests such as hydrogen sulfide, indole, hemolysis, pigment production, etc.
Quality Indicators (COA Test Data)
| Test Item | Unit | Standard | Test Result | Judgment |
|---|---|---|---|---|
| Total Nitrogen | % | ≥ 14.5 | 15.2 | Pass |
| Amino Nitrogen | % | ≥ 2.5 | 3.1 | Pass |
| Sodium Chloride | % | ≤ 5.0 | 2.56 | Pass |
| Loss on Drying (Moisture) | % | ≤ 5.0 | 4.5 | Pass |
| Ash | % | ≤ 5.0 | 3.62 | Pass |
| pH (2% solution) | / | 5.0 – 7.0 | 5.4 | Pass |
| Clarity (2% solution) | / | Clear, no precipitate | Clear, no precipitate | Pass |
| Alkaline Precipitation | / | No precipitate | No precipitate | Pass |
| Appearance / Color | / | Yellow powder | Yellow powder | Pass |
Microbiological Test Performance (Complies with 2000 Biological Products Standard)
| Test Item | Strain | Required Result | Performance |
|---|---|---|---|
| Hydrogen Sulfide Test | Salmonella typhi / Shigella | + / – | Compliant |
| Indole Test | E. coli / S. typhi | + / – | Compliant |
| V‑P Test | Enterobacter aerogenes / E. coli | + / – | Compliant |
| Fermentable Sugar Test | E. coli / S. typhi | No acid production | Compliant |
| Hemolytic Characteristics | Streptococcus pneumoniae / β‑hemolytic Streptococcus | α / β hemolysis | Stable |
| Pigment Production | P. aeruginosa / S. aureus | Green / Golden | Stable |
| Sensitivity Test | Streptococcus / Gram‑positive bacillus | ≥ 10⁻⁴ / 10⁻⁵ | Pass |
Packaging & Storage
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Packaging Specification: 20 kg/bag (inner polyethylene film, outer environmentally friendly woven bag or paper drum)
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Storage Conditions: Cool, dry, ventilated; keep at least 10 cm above the ground and 20 cm away from walls
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Shelf Life: Complies with national regulations; avoid storage with toxic or hazardous substances
Applications
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Biopharmaceutical fermentation (antibiotics, vaccines, enzymes, etc.)
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Microbiological media production (dehydrated media, liquid media)
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Raw material for diagnostic reagents
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Basic nitrogen source for laboratory research
Why Choose Us?
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Complies with Chinese Biological Products Main Raw and Auxiliary Materials Quality Control Standard
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Full testing per batch, traceable data
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Mature process, high batch‑to‑batch stability
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Suitable for demanding microbial culture systems
For more information or customized requirements, please contact us.
Get in Touch
Have questions about our products or want to discuss a custom order? Our team is ready to help you.