Purified Bone Peptone Nitrogenous Substrate for Consistent Nitrogen Base Reproducible Nutrient Support and Controlled Mineral Content
Purified Bone Peptone — Consistent Nitrogen Base for Microbiological Media Applications
Product Overview
Purified Bone Peptone is a nitrogenous substrate manufactured from bovine bone stock using standardized enzymatic hydrolysis procedures. This product provides reproducible nutrient support for microbiological culture media, industrial fermentation processes, and related applications where consistent growth characteristics are required.
Raw Material and Manufacturing
| Element | Description |
|---|---|
| Raw Material Origin | Bovine bone from regulated supply channels |
| Production Sequence | Cleaning → Size reduction → Enzyme treatment → Liquid separation → Concentration → Drying |
| Process Control | Parameter monitoring throughout manufacturing |
| Quality Reference | Enterprise standard Q/SCSH 001-2025 |
Performance Characteristics
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Nitrogen Composition – Total nitrogen meets minimum specification with measurable amino nitrogen fraction
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Solution Properties – Dissolves completely in aqueous systems; solutions maintain clarity through heating cycles
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Mineral Content – Ash and chloride levels controlled to minimize media precipitation
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Physical Attributes – Uniform powder suitable for direct incorporation into formulations
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Lot Uniformity – Manufacturing controls support consistent performance across production batches
Product Specifications
| Parameter | Analytical Method | Unit | Acceptance Limit | Typical Analysis |
|---|---|---|---|---|
| Total Nitrogen | Kjeldahl procedure | % | ≥ 14.5 | 15.2 |
| Amino Nitrogen | Titration with formaldehyde | % | ≥ 2.5 | 3.1 |
| Chloride (as NaCl) | Argentometric titration | % | ≤ 5.0 | 2.6 |
| Moisture | Gravimetric (105°C) | % | ≤ 5.0 | 4.5 |
| Ash | Gravimetric (600°C) | % | ≤ 5.0 | 3.6 |
| pH | 2% aqueous solution | — | 5.0 – 7.0 | 5.4 |
| Physical Form | Visual examination | — | Yellow powder | Conforms |
| Solution Clarity | 2% solution, boiled | — | Clear, no sediment | Conforms |
| Alkaline Stability | pH 8-9, 121°C, 20 min | — | No precipitation | Conforms |
| Nitrite | Colorimetric detection | — | Absent | Conforms |
Microbiological Performance Verification
| Assay | Test Microorganisms | Expected Pattern | Observed Pattern |
|---|---|---|---|
| Hydrogen Sulfide Formation | Salmonella Typhi / Shigella | Positive / Negative | Confirmed |
| Indole Formation | Escherichia coli / Salmonella Typhi | Positive / Negative | Confirmed |
| Acetoin Production | Enterobacter aerogenes / E. coli | Positive / Negative | Confirmed |
| Carbohydrate Reaction | E. coli / Salmonella Typhi | No acid generation | Confirmed |
| Hemolytic Response | S. pneumoniae / β-streptococcus | Alpha / Beta | Consistent |
| Pigment Expression | P. aeruginosa / S. aureus | Green / Golden | Consistent |
| Colony Development | E. coli / S. aureus | ≥1.0mm diameter, smooth | Confirmed |
| Dilution Support | β-streptococcus / Gram-positive bacillus | 10⁻⁴ / 10⁻⁵ | Confirmed |
Application Areas
| Field | Typical Use |
|---|---|
| Industrial Microbiology | Fermentation nutrient base |
| Diagnostic Media | Culture medium component |
| Pharmaceutical Processing | Bacterial cultivation support |
| Research Environments | Media formulation studies |
Packaging Information
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Container Type: Multi-layer bag with inner protective liner
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Net Quantity: 20 kilograms per unit
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Storage Conditions: Dry environment, moderate temperature
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Stability Period: 24 months when stored appropriately
For ordering information or technical inquiries, please contact our customer support team.
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