BACKGROUND INFORMATION

Monkeypox is a viral zoonosis caused by the monkeypox virus that can be transmitted between animals and humans and can also be retransmitted between humans. Based on the current epidemiological investigation, the incubation period is 6 to 13 days, mostly 5 to 21 days. The main symptoms are fever, severe headache, swollen lymph nodes, back pain, muscle aches and weakness. The rash usually begins 1-3 days after a fever and is concentrated on the face and limbs, Lesions can occur at anywhere from a few to thousands of sites, and in severe cases they can combine to cause large chunks of skin to fall off. These rashes are often very painful. When a rash appears, the patient is contagious.

The nucleic acid of Monkeypox infections can be detected via real-time PCR in the specimen of blood and throat swabs, papule or vesicle. The current kit includes a set of specific primers and probes pre-mixed in the Master Mix of the kit targeting two specific gene regions of Monkeypox Virus. The first gene is the orthopoxvirus DNA polymerase gene (E9L) for the detection of Eurasian orthopoxviruses other than variola viruses, and the second one is a viral envelope protein gene (B6R), which specifically detects monkeypox virus (MPXV). A set of primers and probes against human RNase P gene is also included as internal control (IC) for sample collection, processing, and detection.

DETECTION PRINCIPLE

The detection of nucleic acid of Monkeypox Virus is based on quantitative real-time
PCR technology.In RT-PCR reaction, the forward and reverse primers bind to the specific regions of selected genes and amplify the regions (amplicons); the probes specifically bind to target sequence of the amplicons. Specific primers and probes are designed on the specific gene areas of Monkeypox Virus. Probes consist of a reporter fluorophore at 5' and quenching fluorophore at 3'. The fluorescent signals emitted from reporter fluorophores are absorbed by the quenchers, so it doesn’t emit signals. During amplification, probes bound to templates are cut off by Taq enzyme with 5'-3' exonuclease activity, separating reporter dye from the quencher, generating fluorescent signals, the PCR instrument will then automatically draw a real-time amplification curve based on the signal change, realizing the qualitative detection of Monkeypox Virus at the nucleic acid level.

Monkeypox Virus(MPXV) is a disease which is attributed to a virus in the same family of smallpox virus but is not that severe, while it can be deadly to people contract the disease. Early symptoms of monkeypox include a fever, headache, back pain, muscle aches and low energy, then progresses to a rash on the face, hands, feet, eyes, mouth or genitals that turns into raised bumps, or papules.

Huaree Monkeypox Virus Detection Kit, which can quickly identify monkeypox virus infection by specifically testing the nucleic acid fragment of monkeypox virus. This product is aimed to facilitate public health institute worldwide to increase their diagnostic capabilities when facing the risk of Monekeypox virus, allowing for an efficient and rapid public health response in the case of an outbreak.

Features of Monkeypox Virus Detection Kit (PCR-Fluorescent Probe)

◎ Gold standard:real-time polymerase chain reaction (qPCR) assays

◎ Fast and accurately discriminate MPX from other rash illnesses

◎ High sensitivity and specificity

◎ Compatible to mainstream PCR platform used by laboratory.

◎ Affordable price

FAQ:

Q1: Customer service contact information??

WeChat hqz27211, +86 13530382001

Q2: Can you supply samples?

Yes, reasonable quantity samples available to be sent for your evaluation, we can send samples to you by Express(UPS or FedEx). You could prepay the sample cost.

Q3: Can I visit your factory?

Welcome to visit our factory, our factory has professional complete production equipment. We are in Shenzhen, if you want to visit our factory, please contact us in advance.

Q4: Is your company qualified to export to somewhere?

A: YES, we export our products around the world. We have all the certificates (ISO13485, ISO9001, ISO14001, CE, MSDS, EU, America Certificate, Malaysia free sale certificate) required for export and local import custom clearance.

Q5: What's the payment method?

What are the modes of transportation for your products? You can pay USD, EURO & RMB via T/T.We can transport by sea, by train and by air.

Q6: How about the Delivery time?

Samples: about 3-7 days. Mass order: about 30 days after receipt of 30% T/T deposit payment.

Q7: Is your company qualified to export to somewhere?

Yes, we export our products around the world.

Q8: Can you produce according to customers' design?

Yes, we are professional manufacturer; OEM and ODM are both welcomed.

1) Silk print logo on the product;

2) Customized product housing;

3) Customized Color box;

4) Any your Idea on product we can help you to design and put it into production.

Q9: What about your after Sale Service?

1) All products will have been strictly Quality Checked in house before packing.

2) All products will be well packed before shipping.

3) All our products have 2 year warranty, and we sure the product will be free from maintenance within warranty period.

Q10: What about the shipping?

we have strong cooperation with DHL, TNT, UPS, FEDEX, EMS, China Air Post. You also can choose your own shipping forwarder.

Q11: Can you tell me your main customers?

That's our customer's privacy, we should protect their information. At the same time,please rest assured that your information is also safe here.