Theory

This kit is based on colloidal gold immunochromatography and uses double-antigen sandwich assay to detect novel total antibodies & neutralizing antibodies against coronavims in peripheral whole blood.

The N line of the novel coronavirus neutralizing antibody test cassette was coated with recombinant novel coronavims S-RBD antigen, and the C line was coated with sheep anti-S-RBD polyclonal antibody. During detection, the samples were dripped into the sample well, and the neutralizing antibodies of the novel coronavirus was combined with the recombinant S-RBD antigen of the novel coronavirus labeled with colloidal gold, a solid phase the recombinant S-RBD antigen of the novel coronavims - novel coronavims neutralizing antibodies-labelled the recombinant S-RBD antigen of the novel coronavirus - colloidal gold complex at the T line position, solid-phase sheep anti-S-RBD polyclonal antibody - the recombinant S-RBD antigen of the novel coronavinis-colloidal gold complex was formed at the C line.

The T line of the novel coronavims total antibody test cassette was coated with recombinant novel coronavirus nucleoprotein antigen, and the C line was coated with sheep anti-nucleoprotein polyclonal antibody. During detection, the samples were dripped into the sample well, and the total antibodies of the novel coronavims was combined with the recombinant total antigen oi novel coronavims labeled with colloidal gold, a solid phase the recombinant nucleoprotein antigen of the novel coronavirus - novel coronavirus total antibodies - labelled the recombinant nucleoprotein antigen of the novel coronavims - colloidal gold complex at the T line position, solid-phase sheep anti-nucleoprotein polyclonal antibody - the recombinant nucleoprotein antigen of the novel coronavirus-colloidal gold complex was formed at the C line.