Test procedure

- Place the test cassette, sample diluent and test sample for I5~30 minutes, and equilibrate to room temperature (10-30°C).

- Open the aluminum foil pouch of the test cassette, and place the test cassette on a flat surface.

- Write sample ID on the plastic case of the test cassette.

- Peripheral whole blood samples:

• Tear off the disposable alcohol pad, wipe your finger, and dry naturally.

• Using the lancet, pull out the lid of the lancet, press it on the finger that has been disinfected, and puncture the finger with the lancet.

• Squeeze out the first drop of whole blood and wipe it off with a cotton swab.

• Squeeze out the second drop of whole blood, and use a dropper to draw the whole blood above the mark.

• Take 25µl sample (1 drop), add it into the sample well of the test cassette, and add 80 µl(approximately 3 drops) of sample diluent. And then use a cotton swab to stop the bleeding.

• Incubate for 15 minutes at room temperature. Result got after 30minutes is invalid.

- Serum and plasma samples: Take 20µl sample( 1 drop with a dropper), add it into the sample well of the test cassette, and add 80 µl sample diluent (approximately 3 drops with a dropper). Incubate at 10〜30°C for 15 minutes. Result got after 30 minutes is invalid.

- Venous whole blood: Take 25 u L sample (I drop with a dropper), add it into the sample well of the test cassette, and add 80 p L sample diluent (approximately 3 drops with a dropper). Incubate at 10-30°C for 15 minutes. Result got after 30 minutes is invalid.