Based on the principle of specific binding of immunological antigen and antibody, the complex is formed through the antibody recombining with the enzyme labeled chromogenic system, and the enzyme substrate (chromogenic agent) is added to make it chromogenic to locate and characterize the tested antigen. For the positive photos, it is recommended to use formalin to fix the paraffin embedded tonsil tissue section, and the cell staining is located as the nucleus.
Ki-67 protein is a nuclear protein, which is expressed in cell active phase (G1, s, G2 and M phase), but not in quiescent phase (G0); Ki-67 plays an important role in maintaining cell proliferation. Its marker index is related to the process of disease and has prognostic and predictive value.