CMRL Serum Free Cell Culture Medium Contains Amino Acids Vitamins

CMRL medium Serum-free culture of L cells CMRL medium Cell culture medium

product description:

This product contains amino acids, vitamins, inorganic salts, and other ingredients required for various types of cell culture, but does not contain proteins, lipids, or any growth factors, so this product should be used with serum or serum-free supplements.

Product Usage:

CMRL medium was designed by Connaught Medical Research Laboratories and was originally used for serum-free culture of L cells (Earle). After adding horse serum or calf serum, the CMRL medium is especially suitable for the clone culture of monkey kidney cells and the culture of other mammalian cell lines.

Product ingredients:

L-glutamine (Glutamine) is an essential nutrient element in cell culture, but it is unstable in solution and will spontaneously degrade. The medium without L-glutamine can be adjusted arbitrarily according to research needs. - The amount of glutamine, adding fresh L-glutamine or its substitutes is more conducive to the growth of cells when using.

NEAA (non-essential amino acid) is based on the addition of L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline on the basis of MEM medium Amino acid, L-serine, and glycine 7 kinds of NEAA, which can reduce the side effects of non-essential amino acids produced by cells themselves during cell culture, and effectively promote cell proliferation and metabolism.

Glucose can freely adjust the concentration of glucose according to research needs, which is convenient and quick. High-glucose medium is widely used in fast-growing, low-adhesion cells, myeloma cells of hybridomas, cloned cells, transformed cells by DNA transfection, various primary virus host cells, single cell culture, and vaccine production. Production, such as the use of CHO cells to express EPO and produce hepatitis B vaccine. Low glucose medium is more suitable for the culture of slower metabolism, dependent adherent

Precautions:

This product has been sterilized by filtration. Care should be taken during an aseptic operation to avoid contamination.

Do not freeze or thaw the product to keep the product in optimal condition.

This product is for scientific research or further research use only, not for diagnosis or treatment.