Hangzhou AllTest Biotech CO.,LTD
                                                                                                           
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ASFV Antigen Rapid Test Cassette Serum / Plasma / Tissue Porcine Test Kit 10 Minutes

Price Negotiable
Price: negotiation
MOQ: N/A
Delivery Time: 2-4 Weeks
Brand: VECHEK
Product Description

ASFV Antigen Rapid Test Cassette (Serum/Plasma/Tissue),Porcine Test Kit,10 minutes

 

Product: ASFV Antigen Rapid Test Cassette (Serum/Plasma/Tissue),Porcine Test Kit,10 Minutes
Specimen: Serum/Plasma/Tissue
Shelf Life: 2 Years
Reading Time: 10 Minutes
Specificity: >99.99%
Principle: Chromatographic Immunoassay
Pack: 10 T
Format: Cassette
Sensitivity: 98.67%
Accuracy:

99.26%

 

 

Rapid Test Cassette (Serum/Plasma/Tissue) is a rapid chromatographic immunoassay for the qualitative detection of African swine fever virus antigen in serum, plasma or tissue.
 
PRINCIPLE
 
The ASFV Antigen Rapid Test Cassette (Serum/Plasma/Tissue) is based on double antibody sandwich format for the qualitative
detection of African Swine Fever Virus Antigen in serum, plasma or tissue. The test cassette has a testing window. The testin window has T (test) region and C (control) region. The membrane is pre-coated with ASFV antibody. During testing, antigen t ASFV, if present in serum, plasma or tissue specimen, will react with ASFV antibody coated colloidal gold particles in the test strip.
 
The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with ASFV antibody on the membrane in the test line region. If the specimen contains antigen to African Swine Fever Virus, a colored line will appear in the test line region indicating a positive result. If the specimen does not contain African Swine Fever Virus antigen, a colored line will not appear in the test line region indicating a negative result. To serve as a procedural control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.
 
STORAGE AND STABILITY
 
Store as packaged in the sealed pouch either at room temperature or refrigerated (2-30 °C). The test cassette is stable through the expiration date printed on the sealed pouch. The test cassette must remain in the sealed pouch until use. DO NOT FREEZE. Do not use beyond the expiration date.
 
PRECAUTIONS
 
• Do not use after expiration date.
 
• Handle all specimens as if they contain infectious agents. Observe established precautions against microbiological hazards throughout testing and follow the standard procedures for proper disposal of specimens.
 
• Wear disposable gloves and eye protection when specimens are being tested.
 
• Humidity and temperature can adversely affect results.
 
• Do not remove test cassette from its pouch until immediately before use.
 
• Do not reuse the test kit.
 
• Do not mix components from different lot and different products.
 
DIRECTIONS FOR USE
 
Allow the test cassette, specimen, buffer, and/or controls to equilibrate to room temperature (15-30 °C) prior to testing.
Specimen Handling:
 
1. For serum and Plasma: Separate serum or plasma from whole blood as soon as possible to avoid hemolysis. Only use clear, non-hemolyzed specimens. Do not leave the specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8 °C for up to 3 days. For long term storage, specimens should be kept below -20 °C.
 
2. For Tissue: Take a certain amount (rice grain size) of lymphoid tissue samples and mix them evenly with a mixer.
 
Test Reaction:
1. Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is
performed within one hour.
 
2. Place the test cassette on a clean and level surface.
 
• For serum and Plasma:
Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 25 μl) to the specimen well (S) of the test cassette, then quickly add 2 drops of buffer and start the timer. See illustration below.
 
• For Tissue: (lymphoid tissue)
Weigh 0.1 g of the mixed tissue specimens, put them in a specimen collection tube, add 4 drops of buffer, cover the specimen collection tube cap and shake evenly for approximately 10 seconds, then slowly add 2 drops of diluted sample into the specimen well (S) and start the timer. See illustration below.
 
3. Read the results at 10 minutes. Do not interpret results after 20 minutes
 
 
INTERPRETATION OF RESULTS
 
Positive: The presence of both C line and T line, regardless of T line being strong or faint.
Negative: Only clear C line appears.
Invalid: No colored line appears in C zone, regardless of T line’s appearance.

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Company Hangzhou AllTest Biotech CO.,LTD
Location #383, Qiaoxin Road, Xiasha Street, Qiantang Disctrict, Hangzhou, Zhejiang, P.R.China
Contact Person Selina

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