Operation Manual Trichomonas Vaginalis TV RT PCR Detection Kit Lyophilized
Intended use:
Principle:
Main components:
| No. | Components |
BIK-QL-H0020S1 |
|
1 |
PCR Enzyme Mix(Lyophilized) |
24tests/kit |
| 2 | Positive Control | 100 μL/tube |
| 3 | Negative Control | 100 μL/tube |
| 4 | RNase-free water | 1mL/tube |
| 5 |
Paraffin oil |
1.5mL/tube |
Specimen requirements:
1. Specimen type
Urine or female vaginal secretions swab samples.
2. Specimen Preservation
The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃
PCR Amplification condition:
PCR amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 95℃ | 3min |
| 2 | 40 | 95℃ | 10s |
| 60℃ | 30s collect fluorescent |
Instrument detection channel selection: FAM and HEX/VIC.
Quality control:
| Ct value | |
| Negative Control | No Ct |
| Positive Control | ≤30 |
| IC of samples | ≤38 |
Note: Negative Control, Positive Control and IC of samples must be performed correctly, otherwise the sample results is invalid.
Performance indexes of product:
- Accuracy:Use national reference products or enterprise positive/negative reference products for testing, and the results are all positive/negative.
- Detection limitation:1000 copies/mL.
- Precision: The coefficient of variation (CV) of Ct values is≤5%.
- Specificity: The detection results of this kit have no cross reaction with Urealytic Mycoplasma, Mycoplasma hominis, Ureaplasma urealyticum, Mycoplasma pneumoniae,Neisseria gonorrhoeae,Chlamydia trachomatis,herpes simplex virus type Ⅱ,Mycoplasma genitalium.
Operation details please check from IFU!
Get in Touch
Have questions about our products or want to discuss a custom order? Our team is ready to help you.