Biokey UP UU Real Time PCR Detection Kit Lyophilized 96 Tests/Kit

Price Negotiable

Price: USD

MOQ: We can produce liquid and lyophilized kits

Delivery Time: Depending on order quantity

Brand: Biokey

Product Description

UP+UU real-time PCR detection kit (lyophilized)

--96 tests/kit

Intended use:

This kit, adopting Real-Time PCR-Fluorescence technology, is used for detection of Ureaplasma parvum and Ureaplasma urealyticum(UP+UU). It can be used for the laboratory diagnosis and monitoring of UP+UU infection. The test results are only for clinical reference, not for case confirmation or exclusion.

Principle:

The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of UP+UU. The PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument to realize the rapid detection of UP+UU through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.

UP+UU product lyophilization pacakage:

Main components:

No.	Components	BIK-QL-H0022
1	PCR Enzyme Mix (Lyophilized)	96 tests/bottle
2	UP+UU Primer-Probe Mix	100 μL/vial
3	Enzyme Mix Buffer (5×)	400 μL/vial
4	Positive Control	100 μL/tube
5	Negative Control	100 μL/tube
6	RNase-free water	1mL/tube
7	Paraffin oil	1.5mL/tube*2

Note:Do not mix the components from different batches for detection. The Positive Control of UP+UU and internal control were constructed artificially, and they were not infectious.

Specimen requirements:

1. Specimen type

Male urethral secretions or female cervical secretions.

2. Specimen Preservation

The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃

PCR Amplification condition:

PCR amplification according to the following procedures:

Step	Cycle number	Temperature	Time
1	1	95℃	3min
2	40	95℃	10s
2	40	60℃	30s collect fluorescent

Instrument detection channel selection: FAM and HEX/VIC.

Data analysis and interpretation:

	FAM (UP)	VIC/HEX (UU)	ROX (IC)	Interpretation test results
1	-	-	-	Result invalid,test again
2	+	-	+/-	UP(+)
3	-	+	+/-	UU(+)
4	+	+	+/-	UP and UU(+)
5	-	-	+	Negative(-)

NOTE: ‘+’ is positive, ‘-’ is negative.

Operation details please check from IFU!

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Company Guangzhou BioKey Healthy Technology Co.Ltd

Location Add：No.2, Ruitai Rd, Guangzhou Hi-Tech Industrial Development Zone, 510670, CHINA

Contact Person Lisa

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