PRRSV CN Porcine Test Kit Porcine Blue Ear Disease PCR Rapid Test Kit
Porcine reproductive and respiratory disorders syndrome(CN) Nucleic Acid Detection Kit (PCR-Fluorescent Probe Method)
Intended use:
This kit is used for the diagnosis, monitoring and epidemiological investigation of porcine blue ear disease, and is suitable for the specific detection of Porcine Reproductive and respiratory syndrome virus-Chinese strain/highly pathogenic (PRRSV-CN) in pig serum, oral swab, lung lavage fluid, semen, tissue (lung, lymph node, tonsil, spleen, etc.).
Principle:
This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Selection of porcine reproductive and respiratory syndrome virus - China/highly pathogenic strains conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenched group was separated, and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of Porcine reproductive and respiratory syndrome virus-Chinese strain/highly pathogenic nucleic acid in a completely closed reaction system.
Main components:
| Component | Specification |
| PCR reaction liquid | 48 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Sample requirements:
1. Sample type
Pig serum, oral swab, lung lavage fluid, semen, tissue (lung, lymph node, tonsil, spleen, etc.) and other samples
2. Sample collection and transportation
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
The results of interpretation
| Results | Ct Value |
| Positive | Ct≤40 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 40＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Product performance index:
1. Accuracy
The positive reference materials of testing enterprises were all positive.
2. Minimum detection limit
The detection limit of this kit is 3 copies/μL.
3. Precision
Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.
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