Porcine Epidemic Diarrhea virus (PEDV) Nucleic Acid Detection Kit

(PCR-Fluorescent Probe Method)

Intended use:

This kit is used for diagnosis, monitoring and epidemiological investigation of porcine epidemic diarrhea, and is suitable for specific detection of porcine epidemic diarrhea virus (PEDV) in small intestine and feces samples of pigs.

Principle:

This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose the pig epidemic diarrhea virus gene conservative design specific probe, the probe with primer amplification area in the middle of a specific binding DNA template, in the process of

PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of porcine epidemic diarrhea virus was detected by fluorescence PCR in a completely closed reaction system.

Main components:

Sample requirements:

1. Sample type:

Small intestine, feces and other samples of pigs

2. Sample collection and transportation:

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation:

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

The results of interpretation:

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.