Pig Pseudorabies Virus(gB gene) (PRV-gB) Detection Kit , DNA taq polymerase qPCR Probe kit

(PCR-Fluorescent Probe Method)

Intended use:

This kit is used for the diagnosis, monitoring and epidemiological investigation of Pseudorabies Virus(gB gene), and is suitable for the specific detection of PRV-GB gene in pig serum, feces, semen, viscera and other tissue samples.

Principle:

This kit use pig Pseudorabies Virus(gB gene) conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was generated and detected by fluorescence PCR instrument to realize nucleic acid detection of PORcine PSEUDorabies virus gB gene in the completely closed reaction system.

Main components:

Sample requirements:

1. Sample type:

Pig serum, feces, semen, viscera and other tissue samples

2. Sample collection and transportation:

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation:

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

The results of interpretation:

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.