Porcine Circovirus Type 2 Porcine Dna Test Kit PCR Nucleic Acid Test Micgene

Porcine circovirus type 2 (PCV2) Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)

Intended use:

This kit is used for diagnosis, monitoring and epidemiological investigation of porcine circovirus type 2, and is suitable for specific detection of porcine circovirus type 2 (PCV2) in pig serum, feces, semen, viscera and other tissue samples.

Principle:

This kit selection of porcine circovirus virus type 2 conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The porcine circovirus type 2 nucleic acid was detected by fluorescence PCR instrument in a completely closed reaction system.

Main components:

Sample requirements:

1. Sample type:

Pig serum, feces, semen, viscera and other tissue samples

2. Sample collection and transportation:

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation:

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

The results of interpretation:

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.