V. Harveyi(VH) Detection kit Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)
 
Intended use:
 
This kit is suitable for the specific detection of pathogenic Vibrio harveyi (VH) in bait, water samples, shrimp seedlings, shrimp tissues and processed products.
 
Principle:
 
This kit is chosen, V. Harveyi (VH) specific fragment to design primers probe probe, the probe with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Vibrio haviovibrio nucleic acid (VH) was detected by fluorescence PCR instrument in a completely closed reaction system.
 
Main components:
 

 
Sample requirements:
 
1. Sample type:
Shrimp seedling, shrimp tissue, water body, bait, processed products, etc
2. Sample collection and transportation:
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation:
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 
Using Micgene 242/244/244IVD and ABI QuantStudio5:
 
procedures are set as below:

FAM was selected for detection channel.
 
The results of interpretation

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
 
Result analysis:
Automatic analysis using instrumentation
 
Quality control:
Negative control: No obvious amplification curve of FAM detection channel;
Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;
 
The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.