FHV Multiplex Feline Cat Test Kit Stomatitis Real Time PCR Detection Kit FCV FeLV
Multiplex Feline Stomatitis Pathogens real time PCR detection kit(FHV/FCV/FeLV/FIV) nucleic acid detection kit
Intend for Use:
This kit is suitable for the specific detection of feline herpes virus (FHV), feline calicivirus (FCV), feline leukaemia virus (FeLV) and feline AIDS virus (FIV) in cat eye, nose and mouth secretions and EDTA whole blood samples.
Principle:
Under the action of high-efficiency reverse transcriptase, cDNA strands complementary to RNA template were synthesized with PRIMER as starting point and RNA template as template. Choose cat herpes virus, cat goblet virus, feline leukemia virus, HIV gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, In this way, the 3 '-end quenched group is separated, and the fluorescence is emitted, and the nucleic acid detection of feline herpes virus, feline calicivirus, feline leukaemia virus and feline AIDS virus is realized in the completely closed reaction system by fluorescence PCR instrument.
Component:
| Composition | Quantity |
| FHV/FCV PCR liquid | 8 tube |
| FeLV/FIV PCR liquid | 8 tube |
| Positive control | 1 tube |
| Negative control | 1tube |
1. Sample type
Feline eye nasal and mouth secretion and EDTA whole blood samples
2. Sample collection and transportation
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 50℃ | 20min |
| 2 | 1 | 94℃ | 2min30s |
| 3 | 45 | 94℃ | 10s |
| 60℃ | 20s collect fluorescent |
FAM and VIC/HEX were selected for detection channel.
FHV/FCV PCR Reaction reagent:
| FAM channel (FPV) | VIC/HEX channel(FCoV) | Result interpretation | |
| 1 | - | - | Results were reported negative |
| 2 | + | - | Results were reported FHV positive |
| 3 | - | + | Results were reported FCV positive |
| 4 | + | + | Results were reported FHV,FCV positive |
Note: "+" means positive, "-" means negative
FeLV/FIV PCR PCR Reaction reagent:
| FAM channel (TF) | VIC/HEX channel(GD) | Result interpretation | |
| 1 | - | - | Results were reported negative |
| 2 | + | - | Results were reported FeLV positive |
| 3 | - | + | Results were reported FIV positive |
| 4 | + | + |
Results were reported FeLV,FIV positive |
Note: "+" means positive, "-" means negative
Product picture:
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