DAD Detector HPLC Liquid Chromatography Instrument In Quantitative Analysis
DAD Detector Quaternary PumpHigh Performance Liquid Chromatography Instruments in Qualitative and Quantitative Analysis
Description of Liquid Chromatography Instrument
High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure. HPLCinstruments consist of a reservoir of mobilephase, a pump, an injector, a separation column, and adetector. Compounds are separated by injecting a sample mixtureonto the column. The different component in the mixture pass through thecolumn atdifferentiates due to differencesintheirpartition behavior between the mobile phase and thestationary phase. The mobile phase must be degassed to eliminate theformation of air bubbles.
Parameters of Liquid Chromatography Instrument
| DAD Detector Quaternary PumpHigh Performance Liquid Chromatography Instruments in Quantitative Analysis | |
| Physical Specification | |
| Power Requirements | 220V,50HZ |
| Ambient Temperature | 4-40℃ |
| Ambient Humidity | 20-85% relative humidity(non-condensing) |
| System | |
| Qualitative repeatability(Retention time) | RSD≤0.3% |
| Quantitative repeatability(Area) | RSD≤0.6% |
| Pump | |
| Flow Rate Range | 0.0001-10.000mL/min( increments :0.0001 mL/min) |
| Max Operating Pressure | 45MPa |
| Pressure Pulsation | <0.1MPa( Test condition: 10MPa , at 1.0ml/min methanol) |
| Flow Accuracy | ±0.2%( Test condition: 10MPa , at 1.0ml/min methanol) |
| Flow Precision | RSD≤0.06%( Test condition: 10MPa , at 1.0ml/min methanol) |
| Gradient Accuracy | ±1% |
| Column Oven | |
| Temperature Setting Range | 5 °C ~ 85 °C |
| Temperature Stability | ≤0.2°C/h |
| Temperature Controlling Precision | ±0.1 °C |
| Autosampler | |
| Injection Volume Precision |
<0.25%RSD( full loop) ; <0.5%RSD( partial loop) ; <1%RSD( no waste) |
| Linearity | >0.999 |
| Cross Contamination(Carry Over) | <0.01% |
| Sample Capacity | 120 Samples( 1.5ml vials) |
| Syringe | 250 μL( Standard, with 500μL,1000μL, 2500μL optionals) |
| Sample Loop | 20μL( Standard, with 50μL, 100μL, 200μL optional) |
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DAD Detector |
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| Noise | 1.5×10-5Au |
| Drift | ≤1×10-3Au/h |
| LOD | 5.0×10-9g/mL (naphthalene/methanol) |
| Wavelength Range | 190-800nm |
| Wavelength Reproducibility | ≤±0.1 nm |
| Linearity | ≥1.0×104 |
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