Hotstart KnTaq Polymerase SNP Genotyping PCR #P1221
Concentration: 5U/μl
Contents:
| Hotstart KnTaq Polymerase (5U/ul) | 50 μl |
| 10× Taq Buffer (Mg2+ Plus) | 1.25 ml |
Store at -20°C
For research use only.
In total 2 vials.
Description
Hotstart KnTaq Polymerase is an N-truncated Taq DNA polymerase with a stable hot-start system and no 5'-3' exonuclease activity. It is optimized for excellent differentiation of error extensions, and can be used for SNP analysis, genotyping and allele-specific PCR.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Applications
• SNP analysis and genotyping
• Allele-specific PCR
• Multiplexing
• Arbitrarily primed PCR
• Formulation of dried-down amplification reagents
Features
• Thermostable: half-life over 40 min at 95°C incubation
• Generates 3'-dA overhangs PCR products
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