Basic Dye Reticulocyte Stain Solution 2x100mL 6x20mL For Scientific Research

What is Reticulocyte Stain?

Used for staining of reticulocyte and hemoglobin H inclusion body in whole blood. Reticulocyte are transitional cells between young red blood cells and fully mature red blood cells. Due to the presence of basophilic RNA in the cytoplasm of the cells, the cells are stained with reticulocyte staining solution, and the cytoplasm is microscopically examined. A light blue or dark blue mesh structure is visible. Reticulocyte staining solution is mainly used for retinal red blood cell staining. The result of this staining solution is that the background color is very bright and clear, and it is not affected by long-time excessive staining. On the contrary, the longer the staining time, the brighter the background, and it is currently the preferred reticulocyte staining solution.

The reticulated red staining solution was added to the blood, and after incubation at 37℃, hemoglobin H (HbH)

precipitated due to oxidative degeneration, and was in the form of granules, dispersed and evenly dispersed in the red blood cells, and dyed into dark green blue.

Staining Result

• Reticulocyte staining: The cytoplasm of reticulocytes contains a light blue or dark blue network structure, and 1000 red blood cells are observed by oil mirror to calculate the percentage of reticulocytes.
• Hemoglobin H inclusion body staining: In the red blood cells, there are dark green and blue circular bodies of different sizes and numbers, and the irregularities are scattered in the red blood cells. 1000 red blood cells were observed with an oil microscope, and the percentage of HbH inclusion body-positive red blood cells was calculated.

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