Cytology Stains

Basophil Stain (Toluidine blue method) 20 Tests Baso Clear Colourful Stains Testing Principle Toluidine blue has strong heterochromaticity. When acidic mucopolysaccharides are present in the cells, especially sulfur-containing acidic mucous substances, the color of toluidine blue can be changed from blue to red. The basophilic particles in basophils are mainly acid mucopolysaccharides and belong to sulfate esters, which are heterochromatic. Therefore, staining with metachroma

Iron Stain 100 Tests Baso Clear Colourful Stains for Healthcare Facility Testing Principle Normal bone marrow exists in a certain amount of stored iron, in the form of hemosiderin stored in tissue macrophages, available for nucleated red blood cells using synthetic hemoglobin, which exists in red blood cells outside the storage of iron known as extracellular iron, part in the late erythrocyte and a small number of mature red blood cells also contain iron particles, respective

Testing Principle Normal bone marrow exists in a certain amount of stored iron, in the form of hemosiderin stored in tissue macrophages, available for nucleated red blood cells using synthetic hemoglobin, which exists in red blood cells outside the storage of iron known as extracellular iron, part in the late erythrocyte and a small number of mature red blood cells also contain iron particles, respectively, iron granule red blood cells and red iron cells, they belong to

Iron Stain 5 Tests Baso Clear Colourful Stains for Healthcare Facility Testing Principle Normal bone marrow exists in a certain amount of stored iron, in the form of hemosiderin stored in tissue macrophages, available for nucleated red blood cells using synthetic hemoglobin, which exists in red blood cells outside the storage of iron known as extracellular iron, part in the late erythrocyte and a small number of mature red blood cells also contain iron particles, respectively

Testing Principle Naphthol AS-D chloroacetate can be hydrolyzed into Naphthol AS-D by Esterase to mix with diazonium salt and form insoluble red-brown precipitate in cytoplasm. Positive reaction is only found in granulocytes; hence, it is also called “Specific Esterase Stain.” Stain Results Red granules found in cytoplasm means positive. Packaging specification Product Name Ref # Specification Naphthol AS-D Chloroacetate Esterase Stain (AS-DCE) BA4111B 100 Tests/Kit

Testing Principle Naphthol AS-D chloroacetate can be hydrolyzed into Naphthol AS-D by Esterase to mix with diazonium salt and form insoluble red-brown precipitate in cytoplasm. Positive reaction is only found in granulocytes; hence, it is also called “Specific Esterase Stain.” Stain Results Red granules found in cytoplasm means positive. Packaging specification Product Name Ref # Specification Naphthol AS-D Chloroacetate Esterase Stain (AS-DCE) BA4111 20 Tests/Kit

Testing Principle Naphthol AS-D chloroacetate can be hydrolyzed into Naphthol AS-D by Esterase to mix with diazonium salt and form insoluble red-brown precipitate in cytoplasm. Positive reaction is only found in granulocytes; hence, it is also called “Specific Esterase Stain.” Stain Results Red granules found in cytoplasm means positive. Packaging specification Product Name Ref # Specification Naphthol AS-D Chloroacetate Esterase Stain (AS-DCE) BA4111A 5 Tests/Kit

Testing Principle Under alkaline condition, α-Naphthyl Butyrate is hydrolyzed into α-naphthol by Esterase. α-naphthol shall combine with diazonium salt to form insoluble colored precipitate in cytoplasm. This stain has no specificity against esterase; therefore, it is called “Non-specific Esterase Stain”. Stain Results Red or red-brown granules found in cytoplasm means positive. Packaging specification Product Name Ref # Specification a-Naphthyl Butyrate Esterase Stain (a-NBE

Testing Principle Under alkaline condition, α-Naphthyl Butyrate is hydrolyzed into α-naphthol by Esterase. α-naphthol shall combine with diazonium salt to form insoluble colored precipitate in cytoplasm. This stain has no specificity against esterase; therefore, it is called “Non-specific Esterase Stain”. Stain Results Red or red-brown granules found in cytoplasm means positive. Packaging specification Product Name Ref # Specification a-Naphthyl Butyrate Esterase Stain (a-NBE

Testing Principle Under alkaline condition, α-Naphthyl Butyrate is hydrolyzed into α-naphthol by Esterase. α-naphthol shall combine with diazonium salt to form insoluble colored precipitate in cytoplasm. This stain has no specificity against esterase; therefore, it is called “Non-specific Esterase Stain”. Stain Results Red or red-brown granules found in cytoplasm means positive. Packaging specification Product Name Ref # Specification a-Naphthyl Butyrate Esterase Stain (a-NBE

What is a-Naphthyl Acetate Esterase Stain (a-NAE) (Fast blue method) ? α-Naphthyl Acetate is decomposed by esterase and produces α-naphthol, which shall combine with diazonium salt to form dark brown precipitate in cytoplasm. α -NAE is present in monocytes, granulocytes and lymphocytes. It is a kind of neutral non-specific esterase. Therefore, it is also called a nonspecific esterase stain. The positive of mononuclear cells can be inhibited by sodium fluoride, so it is

What is a-Naphthyl Acetate Esterase Stain (a-NAE) (Fast blue method) ? α-Naphthyl Acetate is decomposed by esterase and produces α-naphthol, which shall combine with diazonium salt to form dark brown precipitate in cytoplasm. α -NAE is present in monocytes, granulocytes and lymphocytes. It is a kind of neutral non-specific esterase. Therefore, it is also called a nonspecific esterase stain. The positive of mononuclear cells can be inhibited by sodium fluoride, so it is

What is a-Naphthyl Acetate Esterase Stain (a-NAE) (Fast blue method) ? α-Naphthyl Acetate is decomposed by esterase and produces α-naphthol, which shall combine with diazonium salt to form dark brown precipitate in cytoplasm. α -NAE is present in monocytes, granulocytes and lymphocytes. It is a kind of neutral non-specific esterase. Therefore, it is also called a nonspecific esterase stain. The positive of mononuclear cells can be inhibited by sodium fluoride, so it is

Acid a-Naphthyl Acetate Esterase Stain 100Tests Baso Stains for Medical Diagnosis Detection Principle Hydrochloric acid parafuchsin reacts with Sodium Nitrite to form diazonium salt (six-azo-pararosaniline). α-Naphthyl Acetate is decomposed by esterase and produces α-naphthol, which shall combine with diazonium salt to form red brown precipitate in cytoplasm. This stain has no specificity against esterase; therefore, it is called “Non-specific Esterase Stain.” Stain Results

Acid a-Naphthyl Acetate Esterase Stain 20 Tests Baso Stains for Medical Diagnosis Detection Principle Hydrochloric acid parafuchsin reacts with Sodium Nitrite to form diazonium salt (six-azo-pararosaniline). α-Naphthyl Acetate is decomposed by esterase and produces α-naphthol, which shall combine with diazonium salt to form red brown precipitate in cytoplasm. This stain has no specificity against esterase; therefore, it is called “Non-specific Esterase Stain.” Stain Results

Acid Phosphatase Stain (ACP) 20Tests Baso Clear Colourful Stains What is Acid Phosphatase Stain (ACP)? Acid Phosphatese stain is based on diazotization-coupling principle. At pH5.0, Acid Phosphatase hydrolyzes Naphthol AS-BI Phosphoric Acid and releases Naphthol AS-BI to mix with diazonium salt and form colored precipitate in cytoplasm. Stain Results Mauve granules are positive. Packaging specification Product Name Ref # Specification Acid Phosphatase Stain (ACP) 6 Tests

Acid Phosphatase Stain (ACP) 6 Tests Baso Stains Clear Colourful Stains What is Acid Phosphatase Stain (ACP)? Acid Phosphatese stain is based on diazotization-coupling principle. At pH5.0, Acid Phosphatase hydrolyzes Naphthol AS-BI Phosphoric Acid and releases Naphthol AS-BI to mix with diazonium salt and form colored precipitate in cytoplasm. Stain Results Mauve granules are positive. Packaging specification Product Name Ref # Specification Acid Phosphatase Stain (ACP) 6

What is Neutrophilic Leukocyte Alkaline Phosphatase Stain(NAP)? Neutrophilic-Leukocyte Alkaline Phosphatase stain is based on diazotization-coupling principle. At pH9.2~9.8, Alkaline Phosphatase hydrolyzes Naphthol AS-BI Phosphoric Acid into α-naphthol. The stable diazonium salt and naphthol shall form insoluble colored precipitate in cytoplasm. Stain Results NΑP positive granules are blue. Interpretation Standard: (-)0 point:No positive granules are found in cytoplasm. (+)1

What is Neutrophilic Leukocyte Alkaline Phosphatase Stain(NAP)? Neutrophilic-Leukocyte Alkaline Phosphatase stain is based on diazotization-coupling principle. At pH9.2~9.8, Alkaline Phosphatase hydrolyzes Naphthol AS-BI Phosphoric Acid into α-naphthol. The stable diazonium salt and naphthol shall form insoluble colored precipitate in cytoplasm. Stain Results NΑP positive granules are blue. Interpretation Standard: (-)0 point:No positive granules are found in cytoplasm. (+)1

What is Neutrophilic Leukocyte Alkaline Phosphatase Stain(NAP)? Neutrophilic-Leukocyte Alkaline Phosphatase stain is based on diazotization-coupling principle. At pH9.2~9.8, Alkaline Phosphatase hydrolyzes Naphthol AS-BI Phosphoric Acid into α-naphthol. The stable diazonium salt and naphthol shall form insoluble colored precipitate in cytoplasm. Stain Result NΑP positive granules are blue. Interpretation Standard: (-)0 point:No positive granules are found in cytoplasm. (+)1