Acid Fast Stain (Kinyoun)_TB Stain Baso Clear Colourful Stains

What is Acid Fast Stain (Kinyoun)?

Intended for staining of acid-fast stain of Mycobacteria and other bacteria. This staining kit is based on the Ziehl-Neelsen stain (a carbofulfuchsin solution) recommended by the WHO. Acid-fast bacteria such as Mycobacterium tuberculosis and Mycobacterium leprae are difficult to stain because of their lipoid capsule in the cell wall. However, once the lipoid capsule is stained with an enhanced dye such as carbolfuchsin, the newly formed compound will resist decolorization from acid-alcohol and retain the original color stained (red), which can be easily differentiated from other microorganisms that still adsorb the counter blue stain. 

Staining Result

In the light blue background, acid-fast bacteria (such as: Mycobacterium tuberculosis) was red, the other bacteria and cells were blue.

Mycobacterium tuberculosis most of the rod-shaped, slightly curved; cell width (0.3~0.6) μm; cell length varies between (0.5~8) μm, most in the (1.5~3.5) μm; Older people can be spiral; well-dyed Mycobacterium tuberculosis, can be found in darker colored particles. Fresh specimens containing more Mycobacterium tuberculosis by acid staining, 100× oil lens observation, both to find single bacterium, but also to see clusters clustered or branched arrangement of bacteria.

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