Neutrophilic Leukocyte Alkaline Phosphatase Stain(NAP) 100Tests Baso Stains

What is Neutrophilic Leukocyte Alkaline Phosphatase Stain(NAP)?

Neutrophilic-Leukocyte Alkaline Phosphatase stain is based on diazotization-coupling principle. At pH9.2~9.8, Alkaline Phosphatase hydrolyzes Naphthol AS-BI Phosphoric Acid into α-naphthol. The stable diazonium salt and naphthol shall form insoluble colored precipitate in cytoplasm.

Stain Results

NΑP positive granules are blue.

Interpretation Standard:

(-)0 point:No positive granules are found in cytoplasm.

(+)1 point:Few or sporadic positive granules are found light blue in cytoplasm.

(++)2 points:Medium number of granules are in cytoplasm or blue color appears diffusely.

(+++)3 points:Many granules are in cytoplasm or dark blue color appears diffusely.

(++++)4 points:Full of large granules in cytoplasm or dark blue color appears diffusely.

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