a-Naphthyl Acetate Esterase Stain (a-NAE) (Fast blue method) 6 Tests Baso Clear Colourful Stains

What is a-Naphthyl Acetate Esterase Stain (a-NAE) (Fast blue method) ?

α-Naphthyl Acetate is decomposed by esterase and produces α-naphthol, which shall combine with diazonium salt to form dark brown precipitate in cytoplasm. α -NAE is present in monocytes, granulocytes and lymphocytes. It is a kind of neutral non-specific esterase. Therefore, it is also called a nonspecific esterase stain. The positive of mononuclear cells can be inhibited by sodium fluoride, so it is usually used as sodium fluoride inhibition test as the same time of α-NAE staining.

Stain Results

In the cytoplasm has dark gray or brownish black diffuse or granular precipitation as positive; the nuclei were indigo.

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