Elastic Fiber Stain (Aldehyde fuchsin method) Baso Clear Colourful Stains

Testing Principle

Alkaline fuchsin is mixed with paraformaldehyde. Under the catalysis of hydrochloric acid, paraformaldehyde gradually depolymerizes to release acetaldehyde. The acetaldehyde reacts with the amino groups of the alkaline fuchsia dye to form a deep purple acetaldehyde-alkaline fuchsia complex, known as aldehyde fuchsin. During this process, the solution gradually changes from red to purple, indicating its maturation, and is referred to as aldehyde fuchsin staining solution. Aldehyde fuchsin binds to specific groups in elastic fibers, staining them purple. Additionally, aldehyde fuchsin exhibits strong affinity for certain proteins and sulfated mucopolysaccharides. For example, it can stain mast cell granules, lipofuscin, hepatitis B surface antigen, gastric chief cells, pancreatic islet B cells, and pituitary basophils.

Stain Results

Elastic fibers, mast cell granules, and certain mucins appear purple-red to deep purple, while red blood cells and other tissues exhibit varying shades of yellow.

Packaging Specification