Reverse Transcriptase PCR Reagents

RT - PCR Kit For research use only Cat No. R1011, 20 rxns Cat No. R1012, 100 rxns Components of the kit Component R1011 (20 rxns) R1012 (100 rxns) M-MLV (200U/μl) 20 μl 100 μl RNasin (40U/μl) 12 μl 60 μl Oligo(dT)15 (50μM) 20 μl 100 μl Random hexamer primer (50μM) 20 μl 100 μl 5× First-strand Buffer 80 μl 400 μl RNase-free ddH2O 1 ml 1 ml × 5 dNTPs(10mM each) 50 μl 250 μl Storage All components of the kit should be stored at -20°C. Keep control RNA at -70°C for longer storage

DATP DCTP DGTP DTTP DNTP Mix (RNase Free) R2051 0.5ml dNTP Mix (RNase free) #R2051, 0.5 ml Components Component R2051 0.5ml Concentration 10mM each Store at -20°C For research use only In total 1 vial. Description dNTP Mix (RNase free) is aqueous solutions at pH 7.0 containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10mM. dNTP Mix is ready-to-use solutions designed to save time and to provide higher reproducibility in PCR and other applications. The Mix

RT - PCR Kit For research use only Cat No. R1011, 20 rxns Cat No. R1012, 100 rxns Components of the kit Component R1011 (20 rxns) R1012 (100 rxns) M-MLV (200U/μl) 20 μl 100 μl RNasin (40U/μl) 12 μl 60 μl Oligo(dT)15 (50μM) 20 μl 100 μl Random hexamer primer (50μM) 20 μl 100 μl 5× First-strand Buffer 80 μl 400 μl RNase-free ddH2O 1 ml 1 ml × 5 dNTPs(10mM each) 50 μl 250 μl Storage All components of the kit should be stored at -20°C. Keep control RNA at -70°C for longer storage

Random Hexamer Primer, 6-mer Random Hexamer Primer 6-mer R2031 20μl #R2031, 20 μl Components Component R2031 20μl Concentration 50μM Store at -20°C For research use only In total 1 vial. Description This primer is a mixture of single-stranded random hexanucleotides with 5'- and 3'-hydroxyl ends. The primer is supplied as an aqueous solution and can be used for first strand cDNA synthesis. Applications First strand cDNA synthesis. Quality control Each lot of random hexamer

RT-PCR Mix for qPCR For research use only Cat. No.: R1031 Size: 100 rxns (20 µl reaction system) Components Component R1031 (100 rxns) Enzyme Mix 100 μl 5X RT Buffer 400 μl Control Mix 20 μl RNase-free ddH2O 1 ml × 2 Storage All components should be stored at -20°C, and avoid repeated freezing and thawing. Description RT-PCR Mix for qPCR is a premixed reverse transcription reaction (RT-PCR) kit, easy to operate, can quickly and efficiently complete the reverse transcription

RNasin (RNase Inhibitor) #R2011 1,000U RNasin (RNase Inhibitor) Components Component R2011 (1,000 U) Concentration 40U/μl Store at -20°C For research only In total 1 vial. Description RNasin is a ribonuclease inhibitor purified from human placenta. RNasin reduces the activity of RNase A-type enzymes in a variety of organisms. Source E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor. Storage Buffer 20mM HEPES-NaOH (pH7.5), 50mM NaCl, 8mM DTT, 0.5mM

DEPC-Treated Water R2041/R2042 For research use only DEPC-treated Water, molecular biology grade Cat No # Size R2041 30 ml R2042 100 ml Store at 4°C Description The DEPC-treated Water is deionized, diethylpyrocarbonate (DEPC) treated and 0.22 µm membrane-filtered. It is recommended to be used in any application where RNA is involved. Feature Free of endodeoxyribonucleases, exodeoxyribonucleases, ribonucleases and phosphatases. Applications For use in all molecular biology

Oligo d(T)15 Primer R2021 20μl Oligo d(T)15 Primer #R2021, 20 μl Components Component R2021 20μl Concentration 50μM Store at -20°C For research use only In total 1 vial. Description Oligo d(T)15 Primer is a synthetic single-stranded 15-mer oligonucleotide with 5'- and 3'-hydroxyl ends. This primer is supplied as an aqueous solution and can be used for first strand cDNA synthesis. Applications First strand cDNA synthesis. Quality Control Functionally tested in first strand

RNase Inhibitor Murine R4001 20,000U RNase Inhibitor (Murine) For research use only Components Component R4001 (20,000 U) RNase Inhibitor (Murine) (40 U/μl) 500 μl Storage This reagent should be kept at -20°C. Description RNase Inhibitor (Murine) is a recombinant murine source RNase inhibitor expressed in the soluble form in E. coli. It can inhibit a wide range of RNases (RNase A, B, C). It has been tested by RT-PCR, RT-qPCR and is compatible with various reverse transcriptas

2X One Step RT-PCR Mix Cat. No.: RP1001 Components Component RP1001 (50 rxn, 50 μl/rxn) 2X One Step RT-PCR Mix * 1.25 ml RNase-free ddH2O 1 ml * Contains Reverse Transcriptase, RNase inhibitor, Hotstart Taq DNA Polymerase, dNTPs and buffer Storage This reagent should be kept at -15 ~ -25°C. Description The 2X One Step RT-PCR Mix is used to perform one-step reverse transcription and endpoint PCR applications with any gene-specific primers (GSP) for RNA targets. This master mix

2X One Step RT-PCR Mix Cat. No.: RP1001 Components Component RP1001 (50 rxn, 50 μl/rxn) 2X One Step RT-PCR Mix * 1.25 ml RNase-free ddH2O 1 ml * Contains Reverse Transcriptase, RNase inhibitor, Hotstart Taq DNA Polymerase, dNTPs and buffer Storage This reagent should be kept at -15 ~ -25°C. Description The 2X One Step RT-PCR Mix is used to perform one-step reverse transcription and endpoint PCR applications with any

Gold Reverse Transcriptase R3001(2,000U) Gold Reverse Transcriptase For research use only Components Component R3001 (2,000 U) R3002 (10,000 U) 5 × Gold Buffer 100 μl 500 μl Gold Reverse Transcriptase (200 U/μl) 10 μl 50 μl Storage This reagent should be kept at -30~15°C. Description Gold Reverse Transcriptase is a new reverse transcriptase obtained by in vitro molecular evolution based on M-MLV (RNase H-) Reverse Transcriptase. The first strand of cDNA can be synthesized at

Gold Reverse Transcriptase R3001(2,000U) R3002(10,000U) Gold Reverse Transcriptase For research use only Components Component R3001 (2,000 U) R3002 (10,000 U) 5 × Gold Buffer 100 μl 500 μl Gold Reverse Transcriptase (200 U/μl) 10 μl 50 μl Storage This reagent should be kept at -30~15°C. Description Gold Reverse Transcriptase is a new reverse transcriptase obtained by in vitro molecular evolution based on M-MLV (RNase H-) Reverse Transcriptase. The first strand of cDNA can be

Total RNA TRAzol Reagent R1021 20ml Component R1021 R1022 TRAzol Reagent 20 ml 100 ml TRAzol Reagent TRAzol Reagent is a ready-to-use reagent for the isolation of total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method. During sample homogenization or lysis, TRAzol Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components.

DEPC-Treated Water For research use only DEPC-treated Water, molecular biology grade Cat No # Size R2041 30 ml R2042 100 ml Store at 4°C Description The DEPC-treated Water is deionized, diethylpyrocarbonate (DEPC) treated and 0.22 µm membrane-filtered. It is recommended to be used in any application where RNA is involved. Feature Free of endodeoxyribonucleases, exodeoxyribonucleases, ribonucleases and phosphatases. Applications For use in all molecular biology experiments.

RT-PCR Mix for qPCR RT-PCR Mix for Reverse Transcriptase PCR kit R1031 100 rxns For research use only Cat. No.: R1031 Size: 100 rxns (20 µl reaction system) Components Component R1031 (100 rxns) Enzyme Mix 100 μl 5X RT Buffer 400 μl Control Mix 20 μl RNase-free ddH2O 1 ml × 2 Storage All components should be stored at -20°C, and avoid repeated freezing and thawing. Description RT-PCR Mix for qPCR is a premixed reverse transcription reaction (RT-PCR) kit, easy to operate, can

M-MLV Reverse Transcriptase M-MLV Reverse Transcriptase Rt-PCR R1041 For research use only Components of the kit Component R1041 (5,000U) R1042 (10,000U) M-MLV (200U/μl) 25 μl 50μl 5× First-strand Buffer 100μl 200 μl Description The Dongsheng M-MLV Reverse Transcriptase (RT) is a genetically modified M-MLV RT. It differs from the M-MLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a ribonuclease H

RT-PCR Mix for qPCR For research use only GDSBio RT-PCR Mix for qPCR premixed reverse transcription reaction kit R1031 Cat. No.: R1031 Size: 100 rxns (20 µl reaction system) Components Component R1031 (100 rxns) Enzyme Mix 100 μl 5X RT Buffer 400 μl Control Mix 20 μl RNase-free ddH2O 1 ml × 2 Storage All components should be stored at -20°C, and avoid repeated freezing and thawing. Description RT-PCR Mix for qPCR is a premixed reverse transcription reaction (RT-PCR) kit, easy

M-MLV Reverse Transcriptase High quality M-MLV Reverse Transcriptase Rt-PCR R1042 For research use only Components of the kit Component R1041 (5,000U) R1042 (10,000U) M-MLV (200U/μl) 25 μl 50μl 5× First-strand Buffer 100μl 200 μl Description The Dongsheng M-MLV Reverse Transcriptase (RT) is a genetically modified M-MLV RT. It differs from the M-MLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a

Gold Reverse Transcriptase R3002(10,000U) Gold Reverse Transcriptase For research use only Components Component R3001 (2,000 U) R3002 (10,000 U) 5 × Gold Buffer 100 μl 500 μl Gold Reverse Transcriptase (200 U/μl) 10 μl 50 μl Storage This reagent should be kept at -30~15°C. Description Gold Reverse Transcriptase is a new reverse transcriptase obtained by in vitro molecular evolution based on M-MLV (RNase H-) Reverse Transcriptase. The first strand of cDNA can be synthesized at