MNGS Library Preparation Biological Fluid Sample And Swabs

Host Removal Kit for mNGS Library Preparation in Biological Fluid Sample and Swabs

Samples available for extraction
• This kit is suitable for the extraction and purification of nucleic acids (DNA and RNA) in serum, plasma, virus culture, swab lotion and other samples. Samples should be stored in time after collection to avoid cross-contamination between samples.
• This kit is only used to extract nucleic acid from the sample. The sample types and storage conditions are different. For specific sample requirements, please strictly follow the relevant requirements of nucleic acid testing kit to the samples.
• Before nucleic acid extraction, the sample should be fully mixed to avoid affecting the quantity of nucleic acid extracted.
• When using this kit to extract other samples, please contact our technical service for relevant information.

Company Information

BunnyTeeth Technology Inc. is committed to building a complete core technology platform for bioengineering, building a high-quality biotech product chain and a technical service chain, with the ambition to become a global leader in molecular and genetic diagnostic. BunnyTeeth Technology Inc. will also provide customers with a full-range of one-step service solution in the field of molecular diagnostics and genetic diagnostic.

Product Information

BunnyTeeth Technology Inc. uses a unique unbiased lysis technology that can specifically remove host cells and nucleic acids while retaining intact bacteria, fungi, DNA viruses, chlamydia, mycoplasma and other pathogenic microorganisms in the sample. The enriched microorganisms can be used for various downstream applications such as PCR, qPCR, mNGS after DNA/RNA extraction.

Product Advantages

Various Sample Types: Widely support blood, saliva, alveolar lavage fluid, sputum, swabs and other biological fluid samples.

Unbiased: Unbiased microbial nucleic acid extraction, compatible with multiple pathogen types, minimizes microbial loss in samples.

High Efficiency and Simplicity: Fast and simple operation to minimize the host background and enrich microorganisms efficiently.

Wide Applications: Compatible with a variety of downstream applications, including PCR, qPCR, mNGS, etc., ensuring the accuracy and precision of downstream experiments.

Experimental Data

High Sequencing Abundance and Unbiased Detection of Microbial Fractions

Figure 4. Unbiased DNA extraction for different types of microbiomes.

Z microbial standards were used as test samples into 5 alveolar lavage samples. Host DNA was removed by Host Removal Kit for mNGS Library Preparation in Biological Fluid Sample and Swabs and Zymo similar product and microbial DNA was extracted. Also, library preparation and sequencing were completed. Compared with no host removal, this product was able to efficiently remove host DNA and significantly improve the detection of microorganisms.

Main Composition

Storage Condition

Storage Temperature: 10- 30 ℃ & 2-8 ℃&-20℃

Shelf life: 12 months

Operating Process