Nucleic Acid Covid 19 PCR Test 96-Well Molecular Diagnostic Kit

COVID-19 Nucleic Acid Detection Kit (qRT-PCR) for Molecular Diagnostic with 48-well, 96-well, 192-well, 384-well

Product info

Product description

This kit is intended for use by trained clinical laboratory personnel specifically instructed and trained in the techniques required for performing the test and in vitro diagnostic procedures.

This kit is based on one-step quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) technique. ORF1ab and E gene of novel coronavirus (SRAS-COV-2) are selected as amplification targets, and specific primers and fluorescent labelled probes are designed for the test. ORF1ab gene probe is labeled with FAM fluorescent dye, and E gene probe is labeled with HEX fluorescent dye. After the nucleic acid of clinical samples is extracted, one-step RT-PCR amplification is carried out and fluorescence signals are detected. The instrument software system automatically draws a real-time amplification curve. The human housekeeping RNase P gene is used as the internal control.

Core advantage

Our COVID-19 Nucleic Acid Detection Kit (qRT-PCR) for Molecular Diagnostic with 48-well, 96-well, 192-well, 384-well is targeted at ORF1ab, RNase P and E gene (based upon latest research from US FDA, COVID Omicron has mutation in N genes and S genes, and significantly lower the detection rate of the kits which target on these genes). But so far, there is no evidence to show that E gene has mutation occurred in Omicron. Therefore, our test kit can detect COVID Omicron as efficient as COVID Delta, Alpha, etc.

Parameter

Component

Main Composition: SARS-CoV-2 PCR Reaction Solution, SARS-CoV-2 PCR Enzyme

Mix, Positive Control, Negative Control

Storage condition

The kit should be stored at -20±5℃ in dark and is stable for 12 months. Repeated freeze-thaw cycles should be avoided. See the product label for the date of manufacture and expiration of the kit.

Workflow

1. Reagent Preparation

Remove the kit, melt it at room temperature and mix it with shaking, then centrifuge it at low speed for 10 seconds, and calculate the number of reagents to be prepared for each person (n=number of samples + 2 tubes of control). The reaction system per person was prepared as follows.

Reaction solution preparation table (per person)

Add to an appropriate volume of centrifuge tube, mix thoroughly, and dispense into n PCR reaction tubes in 15µL amounts.

2. Sample Treatment

2.1. Nucleic acid extraction: Take the sample to be tested, the positive control and the negative control, and extract the sample according to the instructions of the nucleic acid extraction kit.

2.2. Sample addition: Add 10uL each of the extracted sample, positive control and negative control to the PCR reaction tubes prepared above, with a final volume of 25uL/ tube, cap tightly and centrifuge instantaneously at low speed.

3. RT-PCR amplification

3.1. Place the reaction tube into the sample slot of the fluorescent PCR amplifier.

3.2. Set the positive control, negative control and sample to be tested in the corresponding order, and set the sample name.

3.3. Select three detection channels, FAM, VIX and ROX. FAM is the ORF1ab indicator channel, VIX is the E gene indicator channel, and ROX is the internal standard RNaseP indicator channel.

3.4. Perform the amplification assay.

Applicable Instrument

Bio-Rad CFX 384 Touch Real-Time PCR Detection System

Applied Biosystems™ Real-Time PCR 7500 System