2000 Tests Qubit DSDNA HS Assay Kit Metagenomic NGS Library Prep Kits

IVDR Certified BunnyQuant dsDNA HS Assay Kit for Sequencing

Company info

BunnyTeeth Technology Inc. is committed to building a complete core technology platform for bioengineering, building a high-quality biotech product chain and a technical service chain, with the ambition to become a global leader in molecular and genetic diagnostic. BunnyTeeth Technology Inc. will also provide customers with a full-range of one-step service solution in the field of molecular diagnostics and genetic diagnostic.

Product info

Product manifestation

IVDR Certified BunnyQuant dsDNA HS Assay Kit for Sequencing is a fluorescent quantification kit for the specific detection of double-stranded DNA (dsDNA). The product is highly selective for dsDNA with good linearity in the 0.2-100 ng range. It can accurately detect dsDNA from various sources including genomic DNA, cell-free DNA, PCR amplification products, sequencing libraries, etc. The detection process is easy, safe and efficient, which is ideal for the quantification of NGS DNA samples.

IVDR Certified BunnyQuant dsDNA HS Assay Kit for Sequencing is a rapid, sensitive and accurate fluorescent quantitative assay for double-stranded DNA (dsDNA).

IVDR Certified BunnyQuant dsDNA HS Assay Kit for Sequencing is used in conjunction with a fluorometric quantification instrument or enzymatic standard for pre-analytical quantification of nucleic acids and allows for accurate quantification of double-stranded DNA from 0.2 ng/μL to 100 ng/μL. Its processed product is used for nucleic acid analysis.

IVDR Certified BunnyQuant dsDNA HS Assay Kit for Sequencing uses a highly selective fluorescent dye to specifically bind the double-stranded DNA in the sample. the fluorescence value of the standard is detected using a fluorescence quantifier or an enzyme standardizer, and a standard curve is produced. The concentration of double-stranded DNA in the sample is then calculated by detecting the fluorescence value of the sample.

Methodology

UV absorption photometry:based on the intrinsic absorption properties of nucleic acids (DNA and RNA) at 260 nm

Fluorescent assays:based on the use of fluorescent dyes that selectively bind DNA, RNA or proteins and emit a signal only when bound to the target

Fluorescence Method

Specificity: differentiated selective measurement of DNA, dsDNA, ssDNA and RNA

More sensitive: can measure pg/mL; especially suitable for very low concentration of nucleic acid samples

Interference resistant: accurate detection of target nucleic acid molecules despite the presence of contaminants (including other nucleic acid contaminants) in the sample

Product advantage

1. Easy Operation: Mix the sample with the fluorescent reagent and after 2 minutes, use Qubit fluorometer or Elisa reader to test.
2. High Sensitivity: The detection limit is lower to 200 pg.
3. Good Tolerance: Tolerate to higher concentrations of salt, urea, ethanol, chloroform, detergents, proteins or agarose and other conventional contaminants.
4. Broad Compatibility: Compatible with DNA quantification from multiple sample sources (plasma, blood, FFPE, tissue, alveolar lavage, swabs, etc.).

Company advantage

1. Production:Full set of reagents are manufactured in GMP production factory with ISO13485 standard; control the source production process, use scientific verification system with industry gold standard
2. Application:Thousands of clinical samples tested in the NGS testing laboratory of Geneseeq (CAP & CLIA certified), with stable results and expected performance indicators

Figure 1-2. High agreement of quantification results with reference standard and competitive product.

Using the IVDR Certified BunnyQuant dsDNA HS Assay Kit for Sequencing and the ThermoFisher relevant kit, 10 dsDNA standards (Lambda DNA) at known concentrations were quantified using Qubit fluorometer and Elisa reader, respectively, and the results of the assay were equivalent to those of the reference standards and the competitor's equivalent products.

Figure 1. Excellent Binding Specificity

Use IVDR Certified BunnyQuant dsDNA HS Assay Kit for Sequencing to quantify dsDNA, RNA, and a mixture of DNA and RNA (1:1). The results show that the product has high binding specificity for dsDNA and not for RNA.

Table 1. Excellent Tolerability to conventional contaminants

Using IVDR Certified BunnyQuant dsDNA HS Assay Kit for Sequencing to quantify dsDNA from the samples containing conventional contaminants (e.g., salts, free nucleotides, proteins, solvents, etc.),the results display that our product has excellent tolerability.

Figure 1. Fast dye binding speed

For 3 dsDNA samples with different concentrations, use IVDR Certified BunnyQuant dsDNA HS Assay Kit for Sequencing to test every 1 min after the 1st min of addition, and record the change in concentration of the samples in 5 min. The product binds dsDNA quickly and reaches saturation within 1 min.

Figure 1. Good repeatability

Use IVDR Certified BunnyQuant dsDNA HS Assay Kit for Sequencing and ThermoFisher similar product to repeat quantification of dsDNA samples at known gradient concentrations separately. This product has good repeatability of quantification and accuracy in line with competitive products..

Figure 1. The DNA quantification results of different samples are in high agreement with competitive products.

Using the IVDR Certified BunnyQuant dsDNA HS Assay Kit for Sequencing and ThermoFisher equivalent for quantification of DNA from different sample types and using a Qubit fluorometer and Elisa reader, respectively, the figure displays that our product is highly consistent with similar products from competing companies.

Component

Main Composition: Lysis Binding Buffer, Washing Buffer 1 & 2, Proteinase K, Superparamagnetic beads

Storage condition

Storage Temperature: 10- 30 ℃,2-8 ℃ & -20 ℃

Shelf life: 12 months

The dsDNA reagent (stored away from light) and standards are stored at -20 ℃, and the 20X buffer is stored at 2-8 ℃. The kit is valid for 1 year.

Operating process