Double-Stranded Genomic DNA High Sensitive Purification Kit for Plasma and Serum

Double-Stranded Genomic DNA High Sensitive Purification Kit

for Plasma and Serum

Company info

BunnyTeeth Technology Inc. is committed to building a complete core technology platform for bioengineering, building a high-quality biotech product chain and a technical service chain, with the ambition to become a global leader in molecular and genetic diagnostic. BunnyTeeth Technology Inc. will also provide customers with a full-range of one-step service solution in the field of molecular diagnostics and genetic diagnostic.

Product info

Product manifestation

Double-Stranded Genomic DNA High Sensitive Purification Kit for Plasma and Serum is a fluorescent quantification kit for the specific detection of double-stranded DNA (dsDNA). The product is highly selective for dsDNA with good linearity in the 0.2-100 ng range. It can accurately detect dsDNA from various sources including genomic DNA, cell-free DNA, PCR amplification products, sequencing libraries, etc. The detection process is easy, safe and efficient, which is ideal for the quantification of NGS DNA samples.

Methodology

UV absorption photometry:based on the intrinsic absorption properties of nucleic acids (DNA and RNA) at 260 nm

Fluorescent assays:based on the use of fluorescent dyes that selectively bind DNA, RNA or proteins and emit a signal only when bound to the target

Product advantage

1. Easy Operation: Mix the sample with the fluorescent reagent and after 2 minutes, use Qubit fluorometer or Elisa reader to test.
2. High Sensitivity: The detection limit is lower to 200 pg.
3. Good Tolerance: Tolerate to higher concentrations of salt, urea, ethanol, chloroform, detergents, proteins or agarose and other conventional contaminants.
4. Broad Compatibility: Compatible with DNA quantification from multiple sample sources (plasma, blood, FFPE, tissue, alveolar lavage, swabs, etc.).

Figure 1. Fast dye binding speed

For 3 dsDNA samples with different concentrations, use Double-Stranded Genomic DNA High Sensitive Purification Kit for Plasma and Serum to test every 1 min after the 1st min of addition, and record the change in concentration of the samples in 5 min. The product binds dsDNA quickly and reaches saturation within 1 min.

Figure 1. Good repeatability

Use Double-Stranded Genomic DNA High Sensitive Purification Kit for Plasma and Serum and ThermoFisher similar product to repeat quantification of dsDNA samples at known gradient concentrations separately. This product has good repeatability of quantification and accuracy in line with competitive products..

Component

Main Composition: Lysis Binding Buffer, Washing Buffer 1 & 2, Proteinase K, Superparamagnetic beads

Storage condition

Storage Temperature: 10- 30 ℃,2-8 ℃ & -20 ℃

Shelf life: 12 months

Operating process