IVDR Nucleic Acid Extraction Magnetic Beads (450mL) for Sanger sequencing, ddPCR, NGS/mNGS Library Preparation

IVDR Nucleic Acid Extraction Magnetic Beads (450mL)

for Sanger sequencing, ddPCR, NGS/mNGS Library Preparation

Company profile

BunnyTeeth Technology Inc. is committed to building a complete core technology platform for bioengineering, building a high-quality biotech product chain and a technical service chain, with the ambition to become a global leader in molecular and genetic diagnostic. BunnyTeeth Technology Inc. will also provide customers with a full-range of one-step service solution in the field of molecular diagnostics and genetic diagnostic.

Product briefing

Product illustration

SPRI (Solid Phase Reverse Immobilization), uses paramagnetic beads to selectively bind nucleic acids by type and size, which can be used to isolate, purify and clean up high purity nucleic acids. The technology can be applied to next-generation sequencing (NGS), Sanger sequencing, qPCR, ddPCR and microarrays, etc. IVDR Nucleic Acid Extraction Magnetic Beads (450mL) for Sanger sequencing, ddPCR, NGS/mNGS Library Preparation uses high performance magnetic beads and a unique buffer system that allows for the adjustment of the ratio of magnetic beads to sample to achieve selection and purification of DNA fragments from 100bp-10kb.

IVDR Nucleic Acid Extraction Magnetic Beads (450mL) for Sanger sequencing, ddPCR, NGS/mNGS Library Preparation are based on the SPRI (Solid Phase Reversible Immobilization) para-magnetic technology, which can selectively bind nucleic acid fragments of different sizes by adjusting the volume ratio between the beads and the sample to meet the needs of different length of nucleic acids, and are suitable for purification and screening of ideal size DNA fragments in high-throughput sequencing. The product is compatible with various brands of DNA and RNA library preparation kits, and can perfectly replace AMPure XP Beads and Axygen Beads, which are widely used in various NGS platforms.

Key characteristics

1. Super Paramagnetic
2. Fast magnetic response
3. High surface area
4. Uniform size
5. Rich carboxyl functional group
6. Low CV

Product performance

1. Accurate, reliable and targeted selection of target fragments
2. Flexible nucleic acid segment screening range to meet different experimental applications
3. Effectively compatible with NGS and other molecular biology processes

Figure 1. The recovery efficiency of magnetic beads is highly consistent with competitive products.

Using different ratios of IVDR Nucleic Acid Extraction Magnetic Beads (450mL) for Sanger sequencing, ddPCR, NGS/mNGS Library Preparation and Ampure XP beads to purify and recover the same amount of DNA fragments, the recovery of IVDR Nucleic Acid Extraction Magnetic Beads (450mL) for Sanger sequencing, ddPCR, NGS/mNGS Library Preparation is highly consistent with Ampure XP beads.

Figure 2. Efficient removal of impurities in cfDNA library preparation and high consistency of purified fragment peak shape with competitive products.

Accurate selection size of fragment

The fragment selection quality is highly consistent with competitive products.

Using BunnyMag, Ampure XP and Axygen beads respectively, IVDR Nucleic Acid Extraction Magnetic Beads (450mL) for Sanger sequencing, ddPCR, NGS/mNGS Library Preparation showed a high degree of consistency with competitive products in terms of fragment selection results under different selection ratio conditions.

Composition

Structure of magnetic beads(3-layer):

Innermost core is Polystyrene (PS)

Second layer wrapped with magnetic material——Fe3O4

The outermost surface is a polymer modified by carboxyl groups(-COOH)

Storage condition

Storage Temperature: 2- 8 ℃

Shelf life: 12 months

Operating procedure