Liquid Biopsy Qubit dsDNA HS Assay Kit NGS Library Preparation (IVDR Certified)

Liquid Biopsy Qubit dsDNA HS Assay Kit

NGS Library Preparation (IVDR Certified)

Company info

BunnyTeeth Technology Inc. is committed to building a complete core technology platform for bioengineering, building a high-quality biotech product chain and a technical service chain, with the ambition to become a global leader in molecular and genetic diagnostic. BunnyTeeth Technology Inc. will also provide customers with a full-range of one-step service solution in the field of molecular diagnostics and genetic diagnostic.

Product info

Product manifestation

Liquid Biopsy Qubit dsDNA HS Assay Kit NGS Library Preparation (IVDR Certified) is a fluorescent quantification kit for the specific detection of double-stranded DNA (dsDNA). The product is highly selective for dsDNA with good linearity in the 0.2-100 ng range. It can accurately detect dsDNA from various sources including genomic DNA, cell-free DNA, PCR amplification products, sequencing libraries, etc. The detection process is easy, safe and efficient, which is ideal for the quantification of NGS DNA samples.

Liquid Biopsy Qubit dsDNA HS Assay Kit NGS Library Preparation (IVDR Certified) is used in conjunction with a fluorometric quantification instrument or enzymatic standard for pre-analytical quantification of nucleic acids and allows for accurate quantification of double-stranded DNA from 0.2 ng/μL to 100 ng/μL. Its processed product is used for nucleic acid analysis.

Liquid Biopsy Qubit dsDNA HS Assay Kit NGS Library Preparation (IVDR Certified) uses a highly selective fluorescent dye to specifically bind the double-stranded DNA in the sample. the fluorescence value of the standard is detected using a fluorescence quantifier or an enzyme standardizer, and a standard curve is produced. The concentration of double-stranded DNA in the sample is then calculated by detecting the fluorescence value of the sample.

Methodology

UV absorption photometry:based on the intrinsic absorption properties of nucleic acids (DNA and RNA) at 260 nm

Fluorescent assays:based on the use of fluorescent dyes that selectively bind DNA, RNA or proteins and emit a signal only when bound to the target

Fluorescence Method

Specificity: differentiated selective measurement of DNA, dsDNA, ssDNA and RNA

More sensitive: can measure pg/mL; especially suitable for very low concentration of nucleic acid samples

Interference resistant: accurate detection of target nucleic acid molecules despite the presence of contaminants (including other nucleic acid contaminants) in the sample

Company advantage

1. Production:Full set of reagents are manufactured in GMP production factory with ISO13485 standard; control the source production process, use scientific verification system with industry gold standard
2. Application:Thousands of clinical samples tested in the NGS testing laboratory of Geneseeq (CAP & CLIA certified), with stable results and expected performance indicators

Experimental data

Figure 1-2. High agreement of quantification results with reference standard and competitive product.

Using the Liquid Biopsy Qubit dsDNA HS Assay Kit NGS Library Preparation (IVDR Certified) and the ThermoFisher relevant kit, 10 dsDNA standards (Lambda DNA) at known concentrations were quantified using Qubit fluorometer and Elisa reader, respectively, and the results of the assay were equivalent to those of the reference standards and the competitor's equivalent products.

Component

Main Composition: Lysis Binding Buffer, Washing Buffer 1 & 2, Proteinase K, Superparamagnetic beads

Storage condition

The dsDNA reagent (stored away from light) and standards are stored at -20 ℃, and the 20X buffer is stored at 2-8 ℃. The kit is valid for 1 year.

Operating process