BunnyTeeth Proteinase K Protocol for DNA/RNA PCR

BunnyTeeth Proteinase K Protocol for DNA/RNA PCR

Product Introduction

BunnyTeeth Proteinase K Protocol for DNA/RNA PCR is a serine protease with efficient enzymatic activity and broad substrate specificity, preferentially breaking down ester and peptide bonds adjacent to the C-terminus of hydrophobic, sulfur-containing, and aromatic amino acids, and is often used to degrade proteins to produce short peptides. It has the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases and has two Ca2+ binding sites around the active center to increase its stability, allowing it to maintain high enzymatic activity under a wider range of conditions.

Technical Features

BunnyTeeth Proteinase K Protocol for DNA/RNA PCR is the colorless to light brown liquid, which concentration is ≥20 mg/mL. Its specific activity is ≥800 U/mL. Both deoxyribonuclease activity and ribonuclease activity are not detected.

Attributes

Application

1. Genetic diagnostic kits;
2. RNA and DNA extraction kits;
3. Extraction of non-protein components from tissues, degradation of protein-like impurities, such as DNA vaccine and heparin preparation;
4. Preparation of chromosomal DNA for pulse electrophoresis;
5. Protein blotting;
6. Development and mass production of enzymatic glycated albumin reagents for IVD reagents.

Precaution

Wear protective gloves and goggles, and keep well ventilated. The product may cause allergic skin reactions.

100 mM buffer solution:

pH 6.0-8.0, Na-phosphate;

pH 8.0-9.0, Tris-HCI;
pH 9.0-12.5,Glycine-NaOH.

Figure 1. Optimum pH

Reaction in 20 mM K-phosphate buffer pH 8.0.
Enzyme concentration:1 mg/mL

Figure 2. Optimum Temperature

25℃ 16 h-treatment with 50 mM buffer solution:

pH 4.5-5.5,Acetate;

pH 6.0-8.0,Na-phosphate;

pH 8.0-9.0, Tris-HC1;

pH 9.5-12.5. Glycine- NaOH.

Enzyme concentration: 1 mg/mL.

Figure 3. pH stability

30 min-treatment with 50 mM Tris HCI buffer, pH 8.0.
Enzyme concentration:1 mg/mL

Figure 4. Thermal stability