Proteinase K Fermentas for Preparation
Proteinase K Fermentas for Preparation
Product Information
Proteinase K Fermentas for Preparation is a serine protease with efficient enzymatic activity and broad substrate specificity, preferentially breaking down ester and peptide bonds adjacent to the C-terminus of hydrophobic, sulfur-containing, and aromatic amino acids, and is often used to degrade proteins to produce short peptides. It has the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases and has two Ca2+ binding sites around the active center to increase its stability, allowing it to maintain high enzymatic activity under a wider range of conditions.
Company Profile
BunnyTeeth Technology Inc. is committed to building a complete core technology platform for bioengineering, building a high-quality biotech product chain and a technical service chain, with the ambition to become a global leader in molecular and genetic diagnostic. BunnyTeeth Technology Inc. will also provide customers with a full-range of one-step service solution in the field of molecular diagnostics and genetic diagnostic.
Technical Features
Proteinase K Fermentas for Preparation is colorless to light brown liquid with the concentration of ≥20 mg/mL. Its specific activity is ≥800 U/mL.
Both deoxyribonuclease activity and ribonuclease activity are not detected.
Attributes
| Source | Tritirachium album | ||
| Classification | EC 3.4.21.64 | ||
| Molecular weight | 29 kDa (SDS-PAGE) | ||
| Isoelectric point | 7.81 | ||
| Optimum pH | 7.0-12.0 all have high activity | Fig. 1 | |
| Temperature | 65 ℃ | Fig. 2 | |
| pH Stability | pH 4.5-12.5 (25 ℃, 16 h) | Fig. 3 | |
| Thermal stability |
Stable below 50 ℃ (pH 8.0, 30min) |
Fig. 4 | |
| Activators | SDS, Urea | ||
| Inhibitors | Di-isopropyl fluorophosphate (DFIP), benzene sulfonyl fluoride (PMSF) | ||
Figure 1. Optimum pH
Figure 2. Optimum Temperature
Figure 3. pH stability
Figure 4. Thermal stability
Application
- Genetic diagnostic kits;
- RNA and DNA extraction kits;
- Extraction of non-protein components from tissues, degradation of protein-like impurities, such as DNA vaccine and heparin preparation;
- Preparation of chromosomal DNA for pulse electrophoresis;
- Protein blotting;
- Development and mass production of enzymatic glycated albumin reagents for IVD reagents.
Storage Conditions
Please store at 2-25 ℃ and the validity period is 12 months.
Precaution
Wear protective gloves and goggles, and keep well ventilated. The product may cause allergic skin reactions.
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