Proteinase K Dilution for Library Preparation and PCR (Liquid)

Proteinase K Dilution for Library Preparation and PCR (Liquid)

Proteinase K Dilution for Library Preparation and PCR (Liquid) is a serine protease with efficient enzymatic activity and broad substrate specificity, preferentially breaking down ester and peptide bonds adjacent to the C-terminus of hydrophobic, sulfur-containing, and aromatic amino acids, and is often used to degrade proteins to produce short peptides. It has the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases and has two Ca2+ binding sites around the active center to increase its stability, allowing it to maintain high enzymatic activity under a wider range of conditions.

Technical Features

Attributes

Experimental Data

Figure 1. Optimum pH

When pH is 7.0-12.0, Proteinase K Dilution for Library Preparation and PCR (Liquid) all have high activity.

Figure 2. Optimum Temperature

Proteinase K Dilution for Library Preparation and PCR (Liquid) has the highest relative activity at the temperature of around 65 ℃.

Figure 3. pH stability

Proteinase K Dilution for Library Preparation and PCR (Liquid) keeps active within pH 4.5-12.5 when it stores at 25 ℃ to16 hours.

Figure 4. Thermal stability

The thermal stability of Proteinase K Dilution for Library Preparation and PCR (Liquid) keeps normal in the condition that the pH is 8.0 and the product is kept at one temperature within 30 minutes.

Application

1. Genetic diagnostic kits;
2. RNA and DNA extraction kits;
3. Extraction of non-protein components from tissues, degradation of protein-like impurities, such as DNA vaccine and heparin preparation;
4. Preparation of chromosomal DNA for pulse electrophoresis;
5. Protein blotting;
6. Development and mass production of enzymatic glycated albumin reagents for IVD reagents.

Precaution

Wear protective gloves and goggles, and keep well ventilated. The product may cause allergic skin reactions.