RNA/DNA Extraction Proteinase K EDTA Buffer for PCR (100mg)

RNA/DNA Extraction Proteinase K EDTA Buffer for PCR (100mg)

Product Description

RNA/DNA Extraction Proteinase K EDTA Buffer for PCR (100mg) is a serine protease with efficient enzymatic activity and broad substrate specificity, preferentially breaking down ester and peptide bonds adjacent to the C-terminus of hydrophobic, sulfur-containing, and aromatic amino acids, and is often used to degrade proteins to produce short peptides. It has the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases and has two Ca2+ binding sites around the active center to increase its stability, allowing it to maintain high enzymatic activity under a wider range of conditions.

Technical Features

Attributes

Experimental Data

Figure 1. Optimum pH

When pH is 7.0-12.0, RNA/DNA Extraction Proteinase K EDTA Buffer for PCR (100mg) all have high activity.

Figure 2. Optimum Temperature

RNA/DNA Extraction Proteinase K EDTA Buffer for PCR (100mg) has the highest relative activity at the temperature of around 65 ℃.

Application

1. Genetic diagnostic kits;
2. RNA and DNA extraction kits;
3. Extraction of non-protein components from tissues, degradation of protein-like impurities, such as DNA vaccine and heparin preparation;
4. Preparation of chromosomal DNA for pulse electrophoresis;
5. Protein blotting;
6. Development and mass production of enzymatic glycated albumin reagents for IVD reagents.

Precaution

Wear protective gloves and goggles, and keep well ventilated. The product may cause allergic skin reactions.