Epstein Barr Virus EBV Real Time PCR Detection Kit Lyophilized 96tests/Kit
Epstein-Barr virus (EBV) real-time PCR detection kit (lyophilized)
--96 tests/kit
Intended use:
This kit, adopting Real-Time PCR-Fluorescence technology, is used for detection of Epstein-Barr virus (EBV). It can be used for the laboratory diagnosis and monitoring of EBV infection. The test results are only for clinical reference, not for case confirmation or exclusion.
Principle:
The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of Epstein-Barr virus (EBV). The PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument to realize the rapid detection of EBV through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.
Main components:
| No. | Components | BIK-QL-H0017 |
|
1 |
PCR Enzyme Mix (Lyophilized) |
96 tests/bottle |
| 2 | EBV Primer-Probe Mix | 100 μL/vial |
| 3 | Enzyme Mix Buffer (5×) | 400 μL/vial |
| 4 | Positive Control | 100 μL/tube |
| 5 | Negative Control | 100 μL/tube |
| 6 | RNase-free water | 1mL/tube |
| 7 | Paraffin oil | 1.5mL/tube*2 |
Note: Do not mix the components from different batches for detection. The Positive Control of EBV and internal control were constructed artificially, and they were not infectious.
Specimen requirements:
1.Specimen type :
Whole blood, serum or plasma.
2.Specimen preservation:
The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃
Reaction mix preparation:
Refer to the tables below to prepare the reaction mix:
| 1×volume required | |
| Resuspended master mix | 14 μL |
|
EBV Primer & probe |
1 μL |
| Total volume | 15 μL |
※Multiply the numbers according to the number of tests.
Quality control:
Negative Control, Positive Control and IC of samples must be performed correctly, otherwise the sample results is invalid.
| Ct value | |
| Negative Control | No Ct |
| Positive Control | ≤30 |
| IC of samples | ≤38 |
PCR amplification condition:
PCR amplification according to the following procedure:
| Step | Cycle | Temperature | Time |
| 1 | 1 | 95℃ | 3 min |
|
2 |
40 |
95℃ | 10 s |
|
60℃ |
30 s (Collection of fluorescence) |
Instrument detection channel selection: FAM and HEX/VIC.
Operation details please check from IFU!
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